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Invitrogen™ IL-8 Human ELISA Kit
Designed to detect and quantify the level of human IL-8 in serum, plasma, and supernatant using 96-well plates and a microplate reader
Brand: Invitrogen™ KHC0081C
2037.75 EUR valid until 2024-12-31
Use promo code "21957" to get your promotional price.
Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Human Interleukin 8 (Hu IL-8) ELISA quantitates Hu IL-8 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-8. Principle of the method The Human IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.Order Info
Shipping Condition: Wet ice
Specifications
| P10145 | |
| <5 pg/mL | |
| ELISA Kit | |
| Human | |
| Colorimetric Microplate Reader | |
| GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1 | |
| 3.9% | |
| HRP | |
| 5 x 96 Tests | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
| Human | |
| 2 hr. 30 min. |
| 15.6-1000 pg/mL | |
| Biotin | |
| Plasma, Serum, Supernatant | |
| Colorimetric | |
| 3576 | |
| 6.1% | |
| Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| <5pg/mL | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |
For Research Use Only. Not for use in diagnostic procedures.