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Macherey-Nagel™ NucleoBond™ PC 10,000 Kit

Purifies up to 10mg of transfection-grade plasmid DNA from E. coli. Macherey-Nagel™ NucleoBond™ PC 10,000 Kit is a classic anion exchanger for fast and easy purification of high- and low-copy plasmids. Complete kit includes giga columns, buffers, RNase A and folder filters.

Brand:  Macherey-Nagel™ 740593


Product Code. 12877521

  • 481.00 € / 5 reactions

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Description

Description

NucleoBond™ kits utilize anion-exchange chromatography with optimized lysis buffer volumes for plasmid DNA purification. PC 10,000 kits are recommended for the isolation of high-copy plasmids (>20 copies/cell). Low-copy plasmids (<20 copies/cell) can be isolated as well, but this requires additional buffers.

  • Folded Filters are included for fast and easy clarification of lysates with no centrifugation required and no shearing forces
  • Contains sufficient buffer to perform 5 giga preps, with typical yields of 2,000–10,000μg of plasmid DNA from E. coli culture
  • Columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range (2.5–8.5) and can remain in contact with buffers for up to three hours
Specifications

Specifications

Yes
Anion Exchange Chromatography Technology
Purification of high-copy and low-copy plasmids
5 Preps
1 Kit
500—2000mL (HC)
120–150 min/2 preps
Gravity-flow Column (Giga)
E. coli Culture
Resuspension Buffer S1 (750mL), Lysis Buffer S2 (750mL), Neutralization Buffer S3 (750mL), Equilibration Buffer N2 (500/125mL), Wash Buffer N3 (1000/125mL), Elution Buffer N5 (500/125mL), RNase A lyophilized (75mg), NucleoBond AX 10000 Columns (5), NucleoBond Folded Filters Type 1 (10), NucleoBond Folded Filters Type 2 (10)
PC 10000 Columns
A260/A280: 1.80–1.95
<300kbp
2,000–10,000μg
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  • Flammable liquid Category 3
  • Substances and mixtures corrosive to metals Category 1
  • Skin corrosion/irritation Category 2
  • Respiratory sensitiser Category 1
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  • Skin sensitiser Category 1

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