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Macherey-Nagel™ NucleoBond™ PC 10,000 Kit
Purifies up to 10mg of transfection-grade plasmid DNA from E. coli. Macherey-Nagel™ NucleoBond™ PC 10,000 Kit is a classic anion exchanger for fast and easy purification of high- and low-copy plasmids. Complete kit includes giga columns, buffers, RNase A and folder filters.
Brand: Macherey-Nagel™ 740593
This item is not returnable.
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Description
NucleoBond™ kits utilize anion-exchange chromatography with optimized lysis buffer volumes for plasmid DNA purification. PC 10,000 kits are recommended for the isolation of high-copy plasmids (>20 copies/cell). Low-copy plasmids (<20 copies/cell) can be isolated as well, but this requires additional buffers.
- Folded Filters are included for fast and easy clarification of lysates with no centrifugation required and no shearing forces
- Contains sufficient buffer to perform 5 giga preps, with typical yields of 2,000–10,000μg of plasmid DNA from E. coli culture
- Columns are resistant to organic solvents and are free of DNase and RNase
- AX Resin can be used over a wide pH range (2.5–8.5) and can remain in contact with buffers for up to three hours
Specifications
| Yes | |
| Anion Exchange Chromatography Technology | |
| Purification of high-copy and low-copy plasmids | |
| 5 Preps | |
| 1 Kit | |
| 500—2000mL (HC) | |
| 120–150 min/2 preps |
| Gravity-flow Column (Giga) | |
| E. coli Culture | |
| Resuspension Buffer S1 (750mL), Lysis Buffer S2 (750mL), Neutralization Buffer S3 (750mL), Equilibration Buffer N2 (500/125mL), Wash Buffer N3 (1000/125mL), Elution Buffer N5 (500/125mL), RNase A lyophilized (75mg), NucleoBond AX 10000 Columns (5), NucleoBond Folded Filters Type 1 (10), NucleoBond Folded Filters Type 2 (10) | |
| PC 10000 Columns | |
| A260/A280: 1.80–1.95 | |
| <300kbp | |
| 2,000–10,000μg |
Safety and Handling
Hazard Category
- Flammable liquid Category 3
- Substances and mixtures corrosive to metals Category 1
- Skin corrosion/irritation Category 2
- Respiratory sensitiser Category 1
- Serious eye damage/eye irritation Category 2
- Skin sensitiser Category 1