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Macherey-Nagel™ NucleoBond™ Xtra Columns
Polypropylene gravity flow columns with inserted column filters for use with NucleoBond Xtra Midi or Maxi kits for ultra-fast isolation of transfection-grade plasmid DNA with high yield.
Brand: Macherey-Nagel™ 740410.10S
Description
NucleoBond™ Xtra—The new generation of anion exchanger for ultra-fast transfection-grade plasmid DNA
- Optimized column design – Midi prep in approximately 30 min, Maxi prep in 35 min
- Available in Midi and Maxi sizes – typical yields of 400 μg (Midi) and 1000 μg (Maxi)
- Polypropylene columns suitable for use with organic solvents and buffers with denaturing agents
- Column filter included for high filter flow rates, parallel lysate clearing, and loading onto the column
- Depth filters avoid time-consuming centrifugation for lysate clearing
- Complete removal of precipitate even with large lysate volumes without clogging
- Avoids shearing of large DNA constructs (such as PACs or BACs)
Specifications
| Room temperature (18 - 25 °C) | |
| Transfection grade DNA | |
| Plasmid DNA purification | |
| Gravity Flow Column | |
| A260/A280: 1.80 - 1.95 | |
| < 300 kbp | |
| 400 μg |
| Anion Exchange Chromatography Technology | |
| NucleoBond Xtra | |
| NucleoBond Xtra Column Filters | |
| 10 Pack | |
| < 200 mL (high copy), < 400 mL (low copy) | |
| 30 min. |
Safety and Handling
Product Identifier
Supplemental information
- NucleoBond Xtra Midi
Supplemental information
- MIXTURE LIST-Contains : 2-propanol/sodium hydroxide solution (diluted < 2 %),dodecylsulfate, sodium salt/ethanol (diluted <