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Macherey-Nagel™ NucleoSpin™ DNA Insect
Combines enzymatic lysis and mechanical disruption of cell walls for efficient isolation of genomic DNA from a wide variety of insects and crustacean samples (fresh, frozen, dried or ethanol preserved). Complete kit includes NucleoSpin™ Bead Tubes.
Brand: Macherey-Nagel™ 740470.50
This item is not returnable.
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Description
Isolation of genomic DNA from insects and crustaceans
- Fast and convenient procedure combining enzymatic lysis and bead disruption
- Suitable for any insect or crustacean (e.g., fruit fly, cricket, mealworm, midge larvae, mosquito)
- High quality DNA from fresh, frozen, dried or ethanol preserved specimen samples
- Bead Tubes included for difficult-to-lyse insects (due to exoskeletons and reinforced cell walls)
- Efficient lysis – Compatible with the most common disruption devices
- Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions
Specifications
| Room temperature (18 - 25 °C) | |
| Silica MembraneTechnology and Mechanical (Bead Tube) Disruption | |
| Binding capacity: 60 μg | |
| Disruption device (e.g. Vortex-Genie 2 with MN Bead Tube Holder), Centrifuge, Vortex mixer | |
| NucleoSpin™ DNA Insect Columns, NucleoSpin™ Bead Tubes Type D, Collection Tubes (2 mL), Buffers | |
| A260/A280: 1.7 - 1.9 | |
| Fragment size: 200 bp - approx. 50 kbp | |
| < 25 μg (varies by sample and disruption device) |
| Mini Spin Column | |
| Insect or Crustacean Specimens (Fresh, Frozen, Dried or Ethanol Preserved) | |
| Elution volume: 25 - 200 μL | |
| Isolation of genomic DNA from insects and crustaceans | |
| 50 | |
| < 40 mg | |
| 35 min/6 prep |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- NucleoSpin DNA Insect
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 2
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
- H411-Toxic to aquatic life with long lasting effects.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
Supplemental information
- MIXTURE LIST-Contains : 1-dodecylpyridiniumchloride, 2-propanol