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Macherey-Nagel™ NucleoSpin™ RNA Plus
State-of-the-art, dual-column kit includes gDNA removal columns and RNA binding columns for efficient and quick RNA isolation from cells and tissues.
Brand: Macherey-Nagel™ 740984.250
This item is not returnable.
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Description
NucleoSpin™ RNA Plus
- Efficient and quick RNA isolation from cells and tissues
- Two-column system includes gDNA removal columns and RNA binding columns
- NucleoSpin™ gDNA Removal Columns – no time consuming rDNase digestion
- Convenient handling – lysate clearing and gDNA removal on one column in one step
- New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary
- Variety of sample materials – cultured cells, animal tissue, plant tissue, microorganisms
- Downstream applications include real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
Specifications
| Yellow/Blue ring | |
| Mini Spin Column | |
| 30 - 120 μL | |
| Binding capacity: 200 μg | |
| Centrifuge | |
| NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers | |
| RNA Isolation Kit | |
| < 30 mg tissue; < 30 mg tissue | |
| HeLa cells (5 x 106): 40-60 μg Mouse liver (20 mg): 80-100 μg Mouse kidney (20 mg): 40-70 μg Mouse spleen (5 mg): 30-60 μg |
| Room temperature (18-25 °C) | |
| Silica Membrane | |
| Cultured Bacterial or Yeast Cells, Human or Animal Tissue | |
| RNA (with typical RIN > 9) | |
| Isolation of RNA | |
| 250 Preps | |
| A260/A280: 1.9 - 2.1 | |
| 20 min/6 preps |
Safety and Handling
Signal Word
Hazard Category
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
Supplemental information
- MIXTURE LIST-Contains : ethyleneglycol diacetate, guanidinium thiocyanate, guanidinehydrochloride, ethanol