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Macherey-Nagel™ NucleoSpin™ TriPrep
NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein
Brand: Macherey-Nagel™ 740966.50
This item is not returnable.
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Description
NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.Superior selectivity - purify your RNA fractionated by size
Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractions. Isolation of total RNA (small and large in one fraction)
Additional isolation of total protein
Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.
Excellent RNA recovery - even without phenol/chloroform
Lysis with chaotropic saltSpin column based procedure
Convenient handling - complete equipment provided
NucleoSpin filters - efficient sample homogenisation. NucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT
- Silica membrane technology
- Format: minispin columns
- Sample size: <107 cultured cells, <30mg human/tissue; <50mg plant tissue, <150μL reaction mixture
- Fragment size, small RNA: 18 to 200 bases
- Fragment size, large RNA: >200 bases
- Typical yield: 10μg small RNA; 95μg large RNA from 107 HeLa cells
- Binding capacity: 200μg
- Elution volume, 30μL to 100μL
- Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
- Small RNA (18 to 200 bases)
- Large RNA (>200 bases)
- Total RNA (small and large RNA in one fraction), and isolate your total protein fraction
- For small and large RNA and total RNA purification, and isolation of total protein fraction.
Specifications
| Shredders | |
| 1 Ea. | |
| Spin Column |
| NucleoSpin TriPrep | |
| With 2mL/1.5mL Collection Tubes, NucleoSpin Filters, buffers, RNase-free rDNase | |
| Rapid purification of total RNA, DNA, and protein from small and precious samples |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
- MIXTURE LIST-Contains : zinc chloride; guanidinium thiocyanate,ethanol (denatured with 1%IPA/1%MEK, acc.2016/1867/EU); rDNa
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