missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. 11944802

OPTIZYME™ AatII, Fisher BioReagents™

Brand:  Fisher BioReagents BP8041-1

Product Code. 11944802

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products »

Explore more special offers
This item is not returnable. View return policy

Description

Description

5'...G A C G T^C...3'
3'...C^T G C A G...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 20 - 50%
  • Buffer 3: 0 - 20%
  • Buffer 4: 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with AatII.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of AatII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Note: Activity decreases if reaction buffer pH is not between 7.5 and 8.0 at 25°C.
  • Compatible Ends: TaiI
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Completely overlaps - blocked
    • GACGTC
  • (Cleavage is blocked)
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - no effect
    • 5'...TGm6ACGTC(N)4 TGCT...3'
  • 3'...AC TGCAG(N)4m6ACGA...5'
  • (No effect on cleavage)

    • TRUSTED_SUSTAINABILITY
    Specifications

    Specifications

    10 U/μL
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with AatII
    Keep container tightly closed
    GACGT.C
    Liquid
    25 to 50% Water, >50% Glycerin
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    300 U
    AatII
    Product Suggestions

    Product Suggestions

    Videos
    Safety and Handling

    Safety and Handling

    Product Identifier
    • OPTIZYME

    SDS
    Documents

    Documents

    Certificates
    Special Offers

    Special Offers

    Product Content Correction

    Your input is important to us. Please complete this form to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.