missing translation for 'onlineSavingsMsg'
Learn More

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

Brand:  Gibco™ A14697

Product Code. 13473269

  • 1330.00 € / 10 reactions

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

  • Used as a Geneticin™ selectable expression plasmid for engineering stable cell lines
  • Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
  • WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
  • TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
  • Neomycin resistance gene for selection of stable cell lines with Geneticin™
  • pUC origin for high copy replication and maintenance of the plasmid in E. coli
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Ampicillin (AmpR)
Constitutive
Transfection
High Copy Plasmid
TA Cloning Kit
Untagged
pUC Origin
NeoR
pcDNA
TOPO™-TA
Store pcDNA™3.4-TOPO™ vector and all cloning reagents at -20°C.
Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C.
Protein Production, Constitutive Expression
TOPO™
CMV
20 μg
Geneticin™ (G-418)
SV40 Promoter
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.