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Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

201.00 € - 2750.00 €

Specifications

GC-Rich PCR Performance High
Polymerase Phusion Plus DNA Polymerase
Reaction Speed 15 to 30 sec./kb
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
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Products 6
Product Code Brand Color No. of Reactions Price Quantity & Availability  
Product Code Brand Color No. of Reactions Price Quantity & Availability  
17278570
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Thermo Scientific™
F632L
Green 500 Reactions
804.00 €
500 reactions
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17288570
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Thermo Scientific™
F632S
Green 100 Reactions
201.00 €
100 reactions
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17208580
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Thermo Scientific™
F632XL
Green 2000 Reactions
2750.00 €
Pack of 4
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17238570
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Thermo Scientific™
F631L
Colorless 500 Reactions
806.00 €
500 reactions
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17248570
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Thermo Scientific™
F631S
Colorless 100 Reactions
202.00 €
100 reactions
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17268570
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Thermo Scientific™
F631XL
Colorless 2000 Reactions
2750.00 €
Pack of 4
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Description

Description

Thermo Scientific Phusion Plus Green PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs. The master mix format simplifies reaction setup with minimal pipetting steps and retains all features of Phusion Plus DNA Polymerase. Additionally, two tracking dyes and a density reagent are pre-added to the master mix for direct loading of PCR products on gels.

Features of Phusion™ Plus PCR Master Mix include:

  • High fidelity—provides >100X higher sequence accuracy than Taq polymerase
  • Convenient setup—enables a universal annealing temperature of 60°C in PCR
  • Enhanced specificity—hot-start mechanism reduces non-specific amplification and primer degradation
  • Benchtop stability—automation enabled due to 24-hour stability of assembled reactions
  • High inhibitor tolerance—works with DNA of suboptimal purity

Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. Its novel reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus PCR Master Mix and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.

Phusion Plus PCR Master Mix is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High-throughput PCR
Specifications

Specifications

High
15 to 30 sec./kb
Hot-start PCR, High-fidelity PCR
>100X
Blunt
20 kb or less
Phusion Plus DNA Polymerase
Dry Ice
2X
Built-In Hot Start
Ready to use master mix
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.