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Thermo Scientific™ Pierce™ Agarose ChIP Kit
Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.
Brand: Thermo Scientific™ 26156
456.28 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.
This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.Highlights:
- Simple and streamlined ChIP assay protocol (< 8 hours)
- Highly efficient isolation and lysis of nuclei
- Easy and reproducible enzymatic digestion
- Low-background and high-capacity Protein A/G agarose resin
- Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
- Fast and reproducible spin-column format; columns and collection tubes included
- High-recovery DNA purification
- Choose a complete ChIP assay kit or the separate chromatin preparation module
Includes:
Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls
Specifications
| >50 mg Human IgG/mL resin binding capacity | |
| Pierce Agarose ChIP Kit | |
| Pierce™ | |
| ChIP Kit | |
| Kit |
| ≥50 mg Human IgG per mL Resin Binding | |
| Sufficient For: 30 complete chromatin IP assay experiments• ChIP Grade Protein A/G Plus Agarose, 0.65 mL (store at 4°C) • IP Dilution/Wash Buffer (5X), 11 mL (store at 4°C) • IP Wash Buffer 3 (5X), 4.5 mL (store at 4°C) • IP Elution Buffer (2X), 4.5 mL (store at 4°C) • Column Accessory Pack, 3 packs (store at room temperature or 4°C) • Microcentrifuge Tubes (1.5mL), 75 tubes (store at room temperature or 4°C) • DNA Clean-Up Column, 40 columns (store at room temperature or 4°C) • DNA Column Binding Solution, 30 mL (store at room temperature or 4°C) • DNA Column Wash Solution, 6 mL (store at room temperature or 4°C) • pH Indicator, 0.8 mL (store at room temperature or 4°C) • DNA Column Elution Solution, 5 mL (store at room temperature or 4°C) • Anti-RNA Polymerase II Antibody, 25 μL (store at -20°C) • Normal Rabbit IgG (1 mg/mL), 10 μL (store at -20°C) • ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL (store at -20°C) • Pierce Chromatin Prep Module (Part No. 26158) (store Proteinase K and Micrococcal Nuclease at -20°C. Store all other components at 4°C). |
|
| 30 Reaction Kit | |
| 30 Reactions |
For Research Use Only. Not for use in diagnostic procedures.