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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation of DYKDDDDK-tagged proteins

Brand:  Thermo Scientific™ A36801

418.90 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.


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Product Code. 15895833

  • 590.00 € / 1mL
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Description

Description

  • The Thermo Scientific Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as “Flag”, is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport.
  • For protein purification or IP, resin is added to a sample containing DYKDDDDK-tagged protein(s) with the tag on either the N- or the C-terminus. Following capture of DYKDDDDK-tagged proteins, non-specifically bound proteins can be washed away before dissociating bound target protein(s) with elution buffer. The Pierce Anti-DYKDDDDK Affinity Resin is recommended for use in spin purification columns or cartridges for FPLC instruments.
  • UltraLink Biosupport is a rigid, hydrophilic, highly crosslinked, copolymeric and porous resin with high coupling capacity. The porosity, rigidity, and durability of the resin also make it suitable for medium-pressure, fast-flow techniques involving large sample volumes.
    • Specific—Unique base-beads and highly specific antibody minimizes off-target binding (low non-specific binding)
    • High purity —Optimized Bind-Wash-Elute protocol enables high purity
    • High Yield —special antibody conjugation method enables high yield
    • Rapid—entire purification protocol typically takes less than 40 mins
    • Economical—purification protocol allows multiple reuses
    • Versatile—can be used in spin-, gravity- or FPLC-based purification and enrichment methods
    • Scalable—available in 1, 5, and 50 mL settled resin package sizes to allow for smaller scale immunoprecipitations or larger scale purifications
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Pierce™
2 mL
Liquid
Wet Ice
Affinity Resin
Affinity Resin
1 mL settled resin. 2 mL supplied as a 50% slurry in PBS containing 0.02% sodium azide. Store at 2–8°C.
Anti-DYKDDDDK monoclonal antibodies
Liquid
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For Research Use Only. Not for use in diagnostic procedures.