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Thermo Scientific™ Pierce™ Boronic Acid Resin
Selectively bind and remove or purify ribonucleotides and small oligonucleotide RNAs using polyacrylamide beads of immobilized m-aminophenyl-boronic acid.
Brand: Thermo Scientific™ 20244
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Description
Perform ribonucleotide and oligonucleotide RNA isolation with Thermo Scientific™ Pierce Boronic Acid Resin.
This immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.Highlights:
Ligand – boronic acid
Support – beaded polyacrylamide (1800 MW exclusion limit)
Spacer – m-aminophenyl group
Loading – 100μmol boronate per milliliter of settled resin
Capacity – at least 99% binding and recovery of 110μmol AMP per milliliter of settled resin
Format – 50% resin slurry in 0.05% sodium azide
Specifications
| Store at 4°C. | |
| Pierce™ | |
| Ribonucleotide | |
| Boronic Acid | |
| Affinity, Polyacrylamide Resin |
| Resin Slurry | |
| 10 mL | |
| Resin | |
| Bottle |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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