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Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit
Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.
129.00 € - 365.00 €
Description
Quantitatively measure lactose dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay with Thermo Scientific™ Pierce LDH Cytotoxicity Assay Kit.
Additional Information and Online Ordering
The Pierce LDH Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays.Highlights:
Convenient – add–mix–read assay format for adherent and suspension cells
Colorimetric – quantitatively measures LDH release by formation of colored product
Robust – uses stable LDH enzyme activity as a cytotoxic marker
Flexible – ideal for high–throughput screening
Non–radioactive – safe alternative to 51Cr–release cytotoxicity assays
Includes:
Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control
Requires:
Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96–well plate compatible with spectrophotometer, multichannel pipette
Recommended for:
- Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
- Detect cytolysis in bioreactor or 3D tissue engineering applications
Specifications
Pierce LDH Cytotoxicity Assay Kit |