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Thermo Scientific™ beta-Galactosidase Assay Reagent
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
149.00 € - 323.00 €
Specifications
Assay | Reporter Enzyme |
---|---|
Content And Storage | Store at -20°C. |
Detection Method | Colorimetric |
Form | Liquid |
For Use With (Application) | Absorbance |
Description
Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit.
The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.Highlights:
- Choose stand-alone assay reagent or complete kit for cell lysis and detection
- Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
- Adaptable to 96-well microplates or tube assay formats
- Perform homogeneous screening assays or measure activity secondarily after cell lysis
- Also compatiible with prepared lysates from other species such as bacteria
Includes:
Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No. 78503), and ready-to-use Stop Solution
Specifications
Reporter Enzyme | |
Colorimetric | |
Absorbance | |
Beta-Gal Substrate | |
Beta-Galactosidase | |
Microplate Reader | |
Pierce beta-Galactosidase Assay Reagent |
Store at -20°C. | |
Liquid | |
Pierce™ | |
Chemical Substrate | |
96-well Plate | |
Enzyme Labeled |
For Research Use Only. Not for use in diagnostic procedures.