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Thermo Scientific™ beta-Galactosidase Assay Reagent

Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

149.00 € - 323.00 €

Specifications

Assay Reporter Enzyme
Content And Storage Store at -20°C.
Detection Method Colorimetric
Form Liquid
For Use With (Application) Absorbance
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Products 2
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Product Code Brand Quantity Price Quantity & Availability  
10638844
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Thermo Scientific™
75705
25 mL
149.00 €
25mL
In Stock
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11849420
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Thermo Scientific™
75710
3 x 25 mL
323.00 €
Pack of 3
Estimated Shipment: 19-11-2024
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Description

Description

Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Highlights:

  • Choose stand-alone assay reagent or complete kit for cell lysis and detection
  • Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
  • Adaptable to 96-well microplates or tube assay formats
  • Perform homogeneous screening assays or measure activity secondarily after cell lysis
  • Also compatiible with prepared lysates from other species such as bacteria

Includes:

Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No. 78503), and ready-to-use Stop Solution

Specifications

Specifications

Reporter Enzyme
Colorimetric
Absorbance
Beta-Gal Substrate
Beta-Galactosidase
Microplate Reader
Pierce beta-Galactosidase Assay Reagent
Store at -20°C.
Liquid
Pierce™
Chemical Substrate
96-well Plate
Enzyme Labeled
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.