Thermo Scientific™ SDAD (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)

Description

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

• Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

• Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

• Reversible - disulfide-containing spacers enable cleavage using reducing agents

• Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

• Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light