Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)

Description

Thermo Scientific™ Pierce PEG12-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 12-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

SPDP reagents have an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a suflhydryl-reactive 2-pyridyldithio group at the opposite end. PEG12-SPDP has a 12-unit polyethylene glycol spacer arm, which confers greater solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using an SPDP reagent.

Highlights:

• Efficient amine conjugation – NHS ester provides effective conjugation to lysines, N-termini of peptides and other primary amines

• Reversible sulfhydryl conjugation – pyridyldithio group enables disulfide linkage with reduced cysteines and other sulfhydryl groups, providing a stable but cleavable bond

• Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and often enhance the solubility of attached molecules

• Spacer arm – connects primary amines and sulfhydryl targets at distances up to 54.1 angstroms

Specifications:

• Chemical name (simplified): 2-pyridyldithiol-tetraoxaoctatriacontane-N-hydroxysuccinimide
• Chemical formula: C39H65N3O17S2
• Molecular weight: 912.07
• Spacer arm: 54.1 angstroms
• Form: light, viscous liquid
• Solubility: dissolve 2mg in 110μL DMSO or DMF to make 20mM stock for each use