MP Biomedicals™ ISIS DNA Polymerase

Description

• High fidelity is a key requirement for many PCR applications and particularly for cloning lengthy and difficult DNA strands. Isis High Fidelity DNA polymerase amplifies long DNA fragments with one of the lowest error rates of the market. Moreover, this polymerase exhibits very high thermal stability, therefore the denaturation temperature can be increased for reading through difficult secondary structure.
• 40 x more accurate than Taq DNA polymerase
• Originally isolated from Pyrococcus abyssi, the Isis DNA Polymerase is a high performant proofreading DNA polymerase. This enzyme has extremely low error rate and is 40 times more accurate than Taq DNA Polymerase, according to the method of Flaman (Flaman, J.M. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 3963-3967). In other terms, it means that the probability of misincorporation is around one error per 1 600 000 base pairs, per PCR cycle.
• Highly thermostable
• The Isis DNA Polymerase is the most thermostable proofreading polymerase available and will therefore retain more activity during PCR, giving more product. 90% of polymerase activity remains after 5 hours incubation at 90°C and 50% after 5 hours at 100°C. Higher thermostability permits greater flexibility in setting denaturation time and temperature, e.g. for difficult, GC rich templates.
• Easy to use
• Isis DNA Polymerase shows very robust activity over a range of different amplification conditions and does not require primer 3′-end protection, unlike many other proofreading enzymes. This means much less time is needed for reaction set-up and optimization.