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R&D Systems™ Recombinant Human HMGB1 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ 1690-HMB-050
469.10 EUR valid until 2024-12-16
Use promo code "21630" to get your promotional price.
This item is not returnable.
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Description
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-12.5 μg/mL.Specifications
| P09429 | |
| Lyophilized from a 0.2 μm filtered solution in PBS. | |
| M.W. (Observed): 28-34 kDa, reducing conditions; M.W. (theoretical): 25 kDa | |
| Reconstitute at 200μg/mL in PBS. | |
| 50 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| Met1 & Gly2 | |
| <0.10 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| Reconstitute at 200 μg/mL in PBS. | |
| HMGB1/HMG-1 |
| Bioactivity | |
| 3146 | |
| HMGB1/HMG-1 | |
| SDS-PAGE: 28-34kDa, reducing conditions | |
| Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein Met1-Glu215 | |
| RUO | |
| Carrier Protein | |
| HMGB1 | |
| Recombinant | |
| >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |