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R&D Systems™ Recombinant Human Wnt-3a Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Brand:  R&D Systems™ 5036-WN-500/CF

4389.52 EUR valid until 2024-12-16
Use promo code "21630" to get your promotional price.


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Product Code. 18694739

  • 5575.00 € / 500µg

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Description

Description

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5.00-25.0 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.
Specifications

Specifications

Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
37.4 kDa
500 μg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Recombinant
89780
Wnt-3a
Chinese Hamster Ovary cell line,CHO-derived human Wnt-3a protein Ser19-Lys352
Unconjugated
75%, by SDS-PAGE with silver staining.
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