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Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Brand: Invitrogen™ A48571
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Description
InvitrogenTM Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Features of the Second Strand cDNA Synthesis Kit include:
- Efficient synthesis of full-length double-stranded cDNA
- Fast—procedure completed in 1 hour
- Convenient—pre-mixed components
- Complete—includes residual RNA removal reagents
Applications
- Full-length double-stranded blunt-end cDNA synthesis for cloning
- Double-stranded cDNA library construction
Specifications
| Contents: • Second Strand Enzyme Mix, 4 x 1 mL • 5X Second Strand Reaction Mix, 4 x 250 μL • 0.5 M EDTA, pH 8.0, 4 x 1 mL • RNase I, 10 U/μL, 4 x 500 μL • Water, nuclease-free, 12 x 1.25 mL Storage: -15 to -25°C |
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| cDNA Synthesis Kit | |
| Double Stranded cDNA | |
| Separate Components |
| 200 reactions | |
| Cloning | |
| 200 Reactions |