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IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.

220.00 € - 286.00 €

Specifications

Format	Liquid
For Use With (Equipment)	StarGate™ System, Mammalian Host Cells
Inducing Agent	SV40 large T antigen (e.g. COS1 or COS7)
pH	8
Promoter	Human CMV Immediate-early

View More Specs

Products 26

Product Code	Brand	Vector	Price	Quantity & Availability
Product Code	Brand	Vector	Price	Quantity & Availability
15653778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4400-001	pESG-IBA wt1	286.00 € 5µg		Please sign in to purchase this item. Need a web account? Register with us today!

15663778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4401-001	pESG-IBA wt2	286.00 € 5µg		Please sign in to purchase this item. Need a web account? Register with us today!

15673778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4403-001	pESG-IBA 3	N/A		N/A	N/A

15683778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4405-001	pESG-IBA 5	N/A		N/A	N/A

15693778 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4423-001	pESG-IBA 23	N/A		N/A	N/A

15603788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4425-001	pESG-IBA 25	N/A		N/A	N/A

15613788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4433-001	pESG-IBA 33	N/A		N/A	N/A

15623788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4435-001	pESG-IBA 35	N/A		N/A	N/A

15633788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4443-001	pESG-IBA 43	N/A		N/A	N/A

15643788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4445-001	pESG-IBA 45	N/A		N/A	N/A

15653788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4462-001	pESG-IBA 62	N/A		N/A	N/A

15663788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4463-001	pESG-IBA 63	N/A		N/A	N/A

15673788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4464-001	pESG-IBA 64	N/A		N/A	N/A

15683788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4465-001	pESG-IBA 65	N/A		N/A	N/A

15693788 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4502-001	pESG-IBA 102	286.00 € 5µg		Please sign in to purchase this item. Need a web account? Register with us today!

15603798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4503-001	pESG-IBA 103	286.00 € 5µg		Please sign in to purchase this item. Need a web account? Register with us today!

15613798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4504-001	pESG-IBA 104	286.00 € 5µg		Please sign in to purchase this item. Need a web account? Register with us today!

15623798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4505-001	pESG-IBA 105	286.00 € 5µg		Please sign in to purchase this item. Need a web account? Register with us today!

15633798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4523-001	pESG-IBA 123	N/A		N/A	N/A

15643798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4542-001	pESG-IBA 142	N/A		N/A	N/A

15653798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4543-001	pESG-IBA 143	N/A		N/A	N/A

15663798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4545-001	pESG-IBA 145	N/A		N/A	N/A

15673798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4562-001	pESG-IBA 162	N/A		N/A	N/A

15683798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4564-001	pESG-IBA 164	N/A		N/A	N/A

15693798 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4567-001	pESG-IBA 167	N/A		N/A	N/A

15603808 View Documents missing translation for 'viewProductCertificates'	IBA Lifesciences 5-4568-001	pESG-IBA 168	N/A		N/A	N/A

Description

pESG-IBA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.

NeoR for generation of stable cells lines.
Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
ColE1 + Amp: for propagation in E. coli
NeoR: neomycin resistance gene to generate stable cell lines
BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Format	Liquid
Inducing Agent	SV40 large T antigen (e.g. COS1 or COS7)
Promoter	Human CMV Immediate-early
Storage Buffer	20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Concentration	250 ng/μL
Color	Clear

For Use With (Equipment)	StarGate™ System, Mammalian Host Cells
pH	8
Product Type	Acceptor Vector for mammalian expression
Content And Storage	2°C to 8°C for frequent usage, -20°C for long-term storage
Quantity	5 μg
For Use With (Application)	Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

Safety and Handling

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IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression

Specifications

Description

Specifications

Safety and Handling

Documents