IBA Solutions For Life Science StarGate™ Upstream Fusion Vector pNFUSE-IBA

Description

StarGate™ offers a simple cloning procedure for rapid and highly efficient subcloning of an arbitrary gene of interest (GOI) for use in functional expression, purification, detection, and immobilization or separation of recombinant proteins.

• Minimal extra modification of the gene due to short combinatorial sites
• Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
• Genes are cloned in a Donor Vector and then parallel-transfered into Acceptor Vectors that provide different genetic surroundings
• Easily connect two or more genes that are cloned in separate Donor Vectors using Fusion Vectors that are assembled by use of a one-tube reaction
• The GOI to be positioned upstream is transferred into a pNFUSE-IBA derivative, specifying the intergenic region (IR):
  • IRES11: Provides internal ribosomal entry site (IRES) from encephalomyocarditis virus for separate cap-independent translation of downstream cistron in mammalian cells
  • SD11: IR includes a Shine Dalgarno (SD) sequence for translation of downstream cistron at comparable level to the upstream cistron in bacterial cells
  • LINK11: Genes of interest are fused by a sequence encoding the 10 amino acid linker GlySerGlyGlyGlySerGlyGlyGlySer
  • LINK12: Genes of interest are fused by a sequence encoding the 15 amino acid linker GlySerGlyGlySerGlyGlyGlyGlySerGlyGlyGlyGlySer
• The GOIs to be positioned downstream are transferred into a pCFUSE-IBA11 vector (identical, irrespective of IR)