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Applied Biosystems™ TaqMan™ Pri-miRNA Assay

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway.

Brand:  Applied Biosystems™ 4427013

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Product Code. 11660129

  • 784.00 € / Each

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Description

Description

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.

Highly specific—quantitiate miRNA transcription from a single genomic locus
Fast, simple, and scalable—two-step RT-qPCR provides high-quality results
A new perspective—measure miRNA expression at the gene level

TaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

Pre-formulated assay (20X; 60X mix)
• 2 unlabeled PCR primers (900 nM each final 1X reaction concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)

TRUSTED_SUSTAINABILITY
Specifications

Specifications

1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

High
Fast
TaqMan™
M (750 reactions), made to order
750 Reactions
60 X
Tube
qPCR
Sustainable packaging
Pri-miRNA Assay
Room Temperature
20X
750 reactions
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For Research Use Only. Not for use in diagnostic procedures.