Thermo Scientific™ pNFkB Tluc16-DD Vector for Luciferase Assays

Description

The Thermo Scientific pMCS Tluc16 Vectors are multiple cloning site (MCS) vectors designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.

• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
• Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another
• Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through
• Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay
• Optimized minimal core promoter (minP) and 5' UTR region for efficient expression of Tluc16 luciferase
• Hygromycin resistance gene for use as selection marker to generate mammalian stable cell lines
• Prepared pNF-κB, and pCRE Tluc16-DD Vectors are designed to monitor the activation of NFκB protein and cAMP-binding protein (CREB) in mammalian cells
• Prepared pEF1α variety includes a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells

Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression. A synthetic polyA terminator and a Transcriptional Pause Site (TPS) are included upstream of the MCS to minimize non-specific transcriptional read-through. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.