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Invitrogen™ uPa/PRAP1 Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHPRAP1
This item is not returnable.
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Description
The Human uPa/PRAP1 ELISA quantitates Hu uPa in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu uPa. Principle of the method The Human uPa solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
May play an important role in maintaining normal growth homeostasis in epithelial cells.
Specifications
| Q96NZ9 | |
| 10 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA Kit | |
| Plasma, Serum, Supernatant | |
| 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 118471 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 96 Tests | |
| Other Research | |
| 2°C to 8°C | |
| Human | |
| 4 hr. 45 min. |
| 13.11-3200 pg/mL | |
| 10pg/mL to 3200pg/mL | |
| Biotin | |
| Other Proteins | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL- 2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL- 15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta1, TGF-beta2, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| Human | |
| Colorimetric Microplate Reader | |
| PRO1195,UPA | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
| RP11-122K13.6, PRO1195, UPA | |
| 1 hr. 20 min. |