Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (13,550)

ELISA Substrates and Reagents (1,222)

Singleplex and Multiplex Bead Based Immunoassays (913)

1 – 15 of 12,839 results

Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

Pricing and Availability

Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

Pricing and Availability
Specifications

Shipping Condition	Ambient
Content And Storage	Store at 2°C to 8°C
Form	Liquid
Product Type	ELISA Stop Solution
Substrate Type	HRP, TMB

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Pricing and Availability
Specifications

Content And Storage	Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Pricing and Availability
Specifications

Content And Storage	Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA

Promotions are available

Pricing and Availability

Invitrogen™ Urea Nitrogen (BUN) Colorimetric Detection Kit

Assay Urea Nitrogen (BUN) levels in Serum, Plasma, Urine, Saliva and Tissue Culture Media.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Colorimetric Detection Kit
Content And Storage	96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at 2–8°C.
For Use With (Equipment)	Microplate Reader
Gene ID (Entrez)	Non-Gene
Gene Symbol	Non-Gene
Target Kit Named	Urea Nitrogen (BUN)

Invitrogen™ Human Albumin ELISA Kit

ELISA

Pricing and Availability
Specifications

Protein Family	Other Proteins
Target Species	Human
Conjugate	HRP
Intraassay CV	<10
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Research Discipline	Other Research
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Assay	Solid-phase sandwich ELISA
Target	Human Serum Albumin
Specificity	This ELISA pair antibody detects human Albumin. Other species not determined.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Calibration Range	60 pg/mL to 12,000 pg/mL
Interassay CV	<12%
Gene Symbol	HSA,PRO0883,PRO0903,PRO1341
Storage Requirements	2°C to 8°C
Assay Range	4.915 to 1200 ng/mL
Accession Number	P02768
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	213
Sample Type	Plasma,Serum,Supernatant
Synonym	GIG20, ANALBA, FDAH, PRO0883, PRO0903, PRO1341

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

Pricing and Availability

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Pricing and Availability
Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	Alkaline Phosphatase
Detection Method	Colorimetric

Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

Pricing and Availability

Invitrogen™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs

Pricing and Availability

Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit

Assay Superoxide Dismutase (SOD) Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Assay Activity Kit
Content And Storage	96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C.
For Use With (Equipment)	Microplate Reader
Target Kit Named	Superoxide Dismutase (SOD)

Invitrogen™ Human VEGF ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.1%
Intraassay CV	4.7
Incubation Time	4 hr.
Gene Symbol	MVCD1,VEGF,VPF
Total Assay Time	4 hrs
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	23.4 to 1500 pg/mL
Marker Gene	VEGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hrs 20 mins
Accession Number	P15692
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	7422
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Human GDF15 ELISA Kit

ELISA

Pricing and Availability
Specifications

Protein Family	Growth Factors & Receptors
Target Species	Human
Conjugate	HRP
Intraassay CV	<10
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Research Discipline	Signal Transduction
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Assay	Solid-phase sandwich ELISA
Target	GDF15
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Calibration Range	2 pg/mL to 800 pg/mL
Interassay CV	<12%
Gene Symbol	GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB
Storage Requirements	2°C to 8°C
Assay Range	1.1 to 800 pg/mL
Accession Number	Q99988
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	9518
Sample Type	Plasma,Serum,Supernatant
Synonym	GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

Invitrogen™ Human FGF-23 ELISA Kit

ELISA

Pricing and Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	FGF23
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.3 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.3 to 75 ng/mL
Test Time	1 hrs 20 mins
Accession Number	ADHR; FGF; Fgf23; FGF-23; FGFN; Fibroblast growth factor; Fibroblast growth factor 23; Fibroblast growth factor 23 C-terminal peptide; Fibroblast growth factor 23 N-terminal peptide; HPDR2; HYPF; Phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor; UNQ3027/PRO9828
Regulatory Status	RUO
Target	FGF23
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	8074
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Immunoassay Reagents and Kits

Immunoassay Reagents and Kits

Thermo Scientific™ Stop Solution for TMB Substrates

Invitrogen™ ELISA Stop Solution

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

Thermo Scientific™ ELISA Carbonate Coating Buffer

Invitrogen™ Urea Nitrogen (BUN) Colorimetric Detection Kit

Invitrogen™ Human Albumin ELISA Kit

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Thermo Scientific™ PNPP Substrates

Invitrogen™ Cell Lysis Buffer II

Invitrogen™ TMB Chromogen Solution (for ELISA)

Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit

Invitrogen™ Human VEGF ELISA Kit

Invitrogen™ Human GDF15 ELISA Kit

Invitrogen™ Human FGF-23 ELISA Kit

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