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Immunoassay Reagents and Kits

Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (11,012)
ELISA Substrates and Reagents (1,222)
Singleplex and Multiplex Bead Based Immunoassays (853)

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1 – 15 of 10,283 results
1

Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

Promotions are available
EDGE
2

Invitrogen™ IL-6 Human ELISA Kit

Measure levels of human interleukin-6 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Promotions are available
EDGE
Measurement Absorbance at 450nm minus absorbance at 550nm
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Specificity No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6
Description Human IL-6 ELISA Kit
Precision Intra-assay CV <10%; inter-assay CV <10%
Total Assay Time 3 hr.
3

Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

Promotions are available
EDGE
4

Invitrogen™ VEGF Human ELISA Kit

Quantifies natural or recombinant human Vascular Endothelial Growth Factor (VEGF) in serum, plasma, buffered solution, or cell culture medium

Promotions are available
EDGE
Target Species Human
Conjugate Biotin
Interassay CV 8.1%
Intraassay CV 4.7%
Incubation Time 4 hr.
Gene Symbol MVCD1,VEGF,VPF
Total Assay Time 4 hr.
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Assay Sensitivity <5 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 23.4-1500 pg/mL
Marker Gene VEGF
For Use With (Application) Colorimetric Detection
Test Time 1 hr. 20 min.
Accession Number P15692
Regulatory Status RUO
Target Human
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 7422
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
5

Invitrogen™ Cortisol Competitive Human ELISA Kit

Competitive ELISA

Promotions are available
Target Species Human
Conjugate HRP
Description Cortisol Competitive ELISA Kit
Interassay CV 8.1%
Intraassay CV 8.8%
Total Assay Time 1 hr. 30 min.
Assay Sensitivity 17.3 pg/mL
Storage Requirements -20°C
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time 1 hr. 20 min.
Detection Method Fluorescence
Regulatory Status RUO
Target Human
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Sample Type Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine
Label or Dye HRP
6

Invitrogen™ Glucose Colorimetric Detection Kit

Assay Glucose levels in Serum, Plasma, Urine, Buffers and Tissue Culture Media.

Promotions are available
Shipping Condition Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format Colorimetric Detection Kit
Content And Storage 96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet.

Store at –20°C.
For Use With (Equipment) Microplate Reader
Product Type Glucose Colorimetric Detection Kit
Gene ID (Entrez) Non-Gene
Sample Type Other Biological Fluids,Plasma,Serum
Gene Symbol Non-Gene
Target Kit Named Glucose
Detection Method Colorimetric
7

Invitrogen™ PSA (Total)/KLK3 Human ELISA Kit

Sandwich ELISA Kit

Protein Family Other Proteins
Target Species Human
Conjugate Biotin
Interassay CV <12%
Intraassay CV <10%
Gene Symbol KLK3
Total Assay Time 4 hr. 45 min.
Assay Sensitivity 8 pg/mL
Storage Requirements 2°C to 8°C
Research Discipline Angiogenesis
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr. 20 min.
Assay Solid-phase sandwich ELISA
Accession Number P07288
Regulatory Status RUO
Target Human
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 354
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
Synonym APS, KLK2A1, PSA, hK3
8

Invitrogen™ Human IL-35 ELISA Kit

For the quantitative detection of Human IL-35

9

Invitrogen™ Thyroxine (T4) Competitive ELISA Kit

Competitive ELISA

Promotions are available
Target Species Human
Conjugate HRP
Description Thyroxine (T4) Competitive ELISA Kit
Interassay CV 7%
Intraassay CV 3%
Total Assay Time 1 hr. 30 min.
Assay Sensitivity 0.29 ng/mL
Storage Requirements -20°C
Research Discipline Metabolism
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time 1 hr. 20 min.
Detection Method Fluorescence
Regulatory Status RUO
Target Chemical
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Sample Type Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye HRP
10

Invitrogen™ Triiodothyronine (T3) Competitive ELISA Kit

Competitive ELISA

Target Species Human
Conjugate HRP
Description Triiodothyronine (T3) Competitive ELISA Kit
Interassay CV 13.4%
Intraassay CV 6.3%
Total Assay Time 2 hr. 30 min.
Assay Sensitivity 37.4 pg/mL
Storage Requirements -20°C
Research Discipline Metabolism
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time 1 hr. 20 min.
Detection Method Fluorescence
Regulatory Status RUO
Target Chemical
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Sample Type Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye HRP
11

Invitrogen™ Corticosterone Competitive ELISA Kit

Competitive ELISA

Promotions are available
Target Species Human
Conjugate HRP
Description Corticosterone Competitive ELISA Kit
Interassay CV 7.9%
Intraassay CV 5.2%
Total Assay Time 1 hr. 30 min.
Assay Sensitivity 18.6 pg/mL
Storage Requirements -20°C
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time 1 hr. 20 min.
Detection Method Fluorescence
Regulatory Status RUO
Target Chemical
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Sample Type Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye HRP
12

Invitrogen™ Catalase Colorimetric Activity Kit

Assay Catalase Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates

Shipping Condition Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format Assay Activity Kit
Content And Storage 96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet.

Store at 2–8°C.
For Use With (Equipment) Microplate Reader
Product Type Catalase Colorimetric Activity Kit
Sample Type Plasma,Serum
Target Kit Named Catalase
Detection Method Colorimetric
13

Invitrogen™ Albumin Human ELISA Kit

Sandwich ELISA Kit

Promotions are available
Protein Family Other Proteins
Target Species Human
Conjugate Biotin
Interassay CV <12%
Intraassay CV <10%
Gene Symbol HSA,PRO0883,PRO0903,PRO1341
Total Assay Time 4 hr. 45 min.
Storage Requirements 2°C to 8°C
Research Discipline Other Research
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr. 20 min.
Assay Solid-phase sandwich ELISA
Accession Number P02768
Regulatory Status RUO
Target Human
Specificity This ELISA pair antibody detects human Albumin. Other species not determined.
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 213
Label or Dye HRP
Synonym GIG20, ANALBA, FDAH, PRO0883, PRO0903, PRO1341
14

Invitrogen™ Interferon alpha (Cell culture) Human ELISA Kit

Measure levels of human interferon alpha (IFNa) in human serum and human plasma samples using 96-well plates.

Measurement Absorbance at 450nm
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Target Species Human
Conjugate HRP
Description Human IFN alpha ELISA Kit (Serum Samples)
Interassay CV <8%
Intraassay CV <8%
Gene Symbol IFNA1,IFNA2
Total Assay Time 3 hr. 15 min.
Sample Volume Supernatant, 100 μL
Storage Requirements 2°C to 8°C
Assay Range 12.5-1000pg/mL
Test Time 1 hr. 20 min.
Regulatory Status RUO
Target Human
Specificity No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t
Precision Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Sample Type Supernatant
Label or Dye HRP
15

Invitrogen™ IL-6 Human ELISA Kit

Key cytokine with actions in immune response

Target Species Human
Conjugate Biotin
Interassay CV 7.9%
Intraassay CV 6.2%
Gene Symbol BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
Total Assay Time 3 hr.
Sample Volume Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Assay Sensitivity <2 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 7.8-2500 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hr. 20 min.
Accession Number P05231
Regulatory Status RUO
Target Human
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision <2pg/mL
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA Kit
Gene ID (Entrez) 3569
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP