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Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Substrate Properties | Chemical Substrate |
---|---|
Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Detection Method | Colorimetric |
Measurement | Absorbance at 450 nm minus absorbance (at 550 nm) |
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Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
Target Species | Human |
Conjugate | HRP |
Description | Human IL-10 ELISA Kit |
Interassay CV | <10% |
Intraassay CV | <10 |
Gene Symbol | CSIF,GVHDS,IL-10 A,TGIF,IL10 |
Total Assay Time | 3 hrs |
Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL |
Assay Sensitivity | <3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 15.36 to 600 pg/mL |
Test Time | 1 hrs 20 mins |
Accession Number | P22301 |
Regulatory Status | RUO |
Target | IL-10 |
Specificity | No significant cross-reaction with human IL-1 A, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10 |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
Precision | Intra-assay CV <10%; inter-assay CV <10% |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA |
Gene ID (Entrez) | 3586 |
Sample Type | Plasma,Serum,Supernatant,Urine |
Label or Dye | HRP |
R&D Systems™ Human VEGF Quantikine ELISA Kit
Human VEGF Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
Specificity | Natural and Recombinant Human VEGF. This assay also recognizes Recombinant Human VEGF165b. |
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Target Species | Human |
Conjugate | HRP |
Gene ID (Entrez) | 7422 |
Sample Type | Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum |
Sample Volume | Cell Culture Supernates (200uL),Serum (100uL),EDTA Plasma (100uL),Heparin Plasma (100uL),Citrate Plasma (100uL) |
Synonym | MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor |
Assay Sensitivity | 5 pg/mL (Cell Culture Supernates), 9 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma) |
Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
Assay Range | 15.60 to 1000 pg/mL (Cell Culture Supernates), 31.30 to 2000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma) |
For Use With (Application) | ELISA |
Cross Reactivity | Cross-species reactivity observed with 1 or more species tested. <0.5% cross-reactivity observed with available related molecules. |
Novus Biologicals™ Human IgG Fc ELISA Kit (Colorimetric)
Human IgG Fc fragment ELISA Kit (Colorimetric) measures IgG Fc fragment in Serum, plasma, cell supernatant, and other biological fluids.
Protein Family | CD Proteins |
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Host Species | Mouse |
Target Species | Mouse |
Conjugate | HRP |
Description | CD36 ELISA Kit, 96 Assays, 1 Kit, Mouse Species, CD Proteins Protein Family, Apoptosis Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Cd36 in Samples, Using a Microplate Reader |
Intraassay CV | <10 |
Detectable Analytes | CD36 |
Substrate Type | TMB |
Total Assay Time | 4 hrs 45 mins |
Sample Volume | Plasma 33 μL, Serum 33 μL, Supernatant 100 μL |
Assay Sensitivity | 30 pg/mL |
Research Discipline | Apoptosis |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hrs 20 mins |
Detection Method | Colorimetric |
Assay | Solid-phase sandwich ELISA |
Target | CD36 |
Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Label or Dye | HRP |
Interassay CV | <12% |
Gene Symbol | FAT,GPIV,Scarb3 |
Storage Requirements | 2°C to 8°C |
Assay Range | 27.43 to 20,000 pg/mL |
Accession Number | Q08857 |
Regulatory Status | RUO |
Product Type | ELISA |
Gene ID (Entrez) | 12491 |
Sample Type | Plasma,Serum,Supernatant |
Synonym | FAT, GPIV, Scarb3 |
Protein Family | Chemokine & Receptors |
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Host Species | Mouse |
Target Species | Mouse |
Conjugate | HRP |
Description | HGF ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Chemokines and Receptors Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of HGF in Samples |
Intraassay CV | <10 |
Detectable Analytes | HGF |
Total Assay Time | 4 hrs 45 mins |
Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
Assay Sensitivity | 400 pg/mL |
Research Discipline | Apoptosis |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hrs 20 mins |
Detection Method | Colorimetric |
Assay | Solid-phase sandwich ELISA |
Target | HGF |
Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Label or Dye | HRP |
Calibration Range | 400 pg/mL to 60 ng/mL |
Interassay CV | <12% |
Gene Symbol | C230052L06Rik,HGF/SF,NK1,NK2,SF,SF/HGF |
Storage Requirements | 2°C to 8°C |
Assay Range | 0.614 to 60 ng/mL |
Accession Number | Q08048 |
Regulatory Status | RUO |
Product Type | ELISA |
Gene ID (Entrez) | 15234 |
Sample Type | Plasma,Serum,Supernatant |
Synonym | C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF |
Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
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Description | Human IL-6 ELISA Kit |
Precision | Intra-assay CV <10%; inter-assay CV <10% |
Protein Family | Chemokine & Receptors |
---|---|
Host Species | Mouse |
Target Species | Mouse |
Conjugate | HRP |
Intraassay CV | <10 |
Detectable Analytes | CTLA-4 |
Substrate Type | TMB |
Total Assay Time | 4 hrs 45 mins |
Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
Assay Sensitivity | 5 pg/mL |
Research Discipline | Immunology |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hrs 20 mins |
Detection Method | Colorimetric |
Assay | Solid-phase sandwich ELISA |
Target | CTLA-4 (CD152) |
Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL- 17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Label or Dye | HRP |
Calibration Range | 5 pg/mL to 1200 pg/mL |
Interassay CV | <12% |
Gene Symbol | Cd152,Ctla-4,Ly-56 |
Storage Requirements | 2°C to 8°C |
Assay Range | 4.92 to 1200 pg/mL |
Accession Number | P09793 |
Regulatory Status | RUO |
Product Type | ELISA |
Gene ID (Entrez) | 12477 |
Sample Type | Plasma,Serum,Supernatant |
Synonym | CH29-120A16.2, Cd152, Ctla-4, Ly-56 |
Protein Family | Chemokine & Receptors |
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Host Species | Mouse |
Target Species | Mouse |
Conjugate | HRP |
Description | BLC (CXCL13) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of BLC (CXCL13) in Samples Using a Microplate Reader |
Intraassay CV | <10 |
Detectable Analytes | BLC (CXCL13), mouse BLC |
Total Assay Time | 4 hrs 45 mins |
Sample Volume | Plasma 5 μL, Serum 5 μL, Supernatant 100 μL |
Assay Sensitivity | 2 pg/mL |
Research Discipline | Inflammation |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hrs 20 mins |
Detection Method | Colorimetric |
Assay | Solid-phase sandwich ELISA |
Target | CXCL13 |
Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Label or Dye | HRP |
Calibration Range | 2 pg/mL to 2000 pg/mL |
Interassay CV | <12% |
Gene Symbol | Cxcl13 |
Storage Requirements | 2°C to 8°C |
Assay Range | 1.37 to 1000 pg/mL |
Accession Number | O55038 |
Regulatory Status | RUO |
Product Type | ELISA |
Gene ID (Entrez) | 55985 |
Sample Type | Plasma,Serum,Supernatant |
Synonym | 4631412M08Rik, ANGIE2, Angie, BCA-1, BLC, BLR1L, Scyb13 |
Protein Family | Proteases, Peptidases & Inhibitors |
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Host Species | Mouse |
Target Species | Mouse |
Conjugate | HRP |
Description | DPPIV (DPP4) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Proteases, Peptidases and Inhibitors Protein Family, Cell Cycle and Proliferation Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Dppiv (Dpp4) i |
Intraassay CV | <10 |
Detectable Analytes | DPPIV (DPP4) |
Substrate Type | TMB |
Total Assay Time | 4 hrs 45 mins |
Sample Volume | Plasma 5 μL, Serum 5 μL, Supernatant 100 μL |
Assay Sensitivity | 0.45 ng/mL |
Research Discipline | Cell Cycle & Proliferation |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hrs 20 mins |
Detection Method | Colorimetric |
Assay | Solid-phase sandwich ELISA |
Target | DPP4 |
Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Label or Dye | HRP |
Calibration Range | 0.45 ng/mL to 100 ng/mL |
Interassay CV | <12% |
Gene Symbol | Cd26,Dpp-4,THAM |
Storage Requirements | 2°C to 8°C |
Assay Range | 0.41 to 100 ng/mL |
Accession Number | P28843 |
Regulatory Status | RUO |
Product Type | ELISA |
Gene ID (Entrez) | 13482 |
Sample Type | Plasma,Serum,Supernatant |
Synonym | RP23-87G8.2, Cd26, Dpp-4, THAM |
Protein Family | Chemokine & Receptors |
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Host Species | Mouse |
Target Species | Mouse |
Conjugate | HRP |
Description | 6-Ckine (CCL21A) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of 6-Ckine (CCL21A) in Samples Using a Microplate Reader |
Intraassay CV | <10 |
Detectable Analytes | 6-Ckine (CCL21A) |
Total Assay Time | 4 hrs 45 mins |
Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
Assay Sensitivity | 20 pg/mL |
Research Discipline | Inflammation |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hrs 20 mins |
Detection Method | Colorimetric |
Assay | Solid-phase sandwich ELISA |
Target | CCL21 |
Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Label or Dye | HRP |
Interassay CV | <12% |
Gene Symbol | 6 CKBAC2,6 Ckine,ALP,AW987545,CKb9,SCYA21a,SLC,Scya21,Scya21b,Tca4,plt |
Storage Requirements | 2°C to 8°C |
Assay Range | 31.25 to 1000 pg/mL |
Accession Number | P84444 |
Regulatory Status | RUO |
Product Type | ELISA |
Gene ID (Entrez) | 100042493, 18829, 65956 |
Sample Type | Plasma,Serum,Supernatant |
Synonym | RP23-411B7.4, 6 CKBAC2, 6 Ckine, ALP, AW987545, CKb9, SCYA21a, SLC, Scya21, Scya21b, Tca4, plt |
Target Species | Human |
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Conjugate | HRP |
Description | Progesterone Competitive ELISA Kit |
Interassay CV | 5.7% |
Intraassay CV | 3.9 |
Total Assay Time | 2 hrs 30 mins |
Sample Volume | Fecal Extract 50 μL, Supernatant 50 μL, Urine 12.5 μL |
Assay Sensitivity | 47.9 pg/mL |
Storage Requirements | -20°C |
Research Discipline | Reproductive Biology |
Assay Range | 50 to 3200 pg/mL |
For Use With (Application) | ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti |
Test Time | 1 hrs 20 mins |
Detection Method | Fluorescence |
Regulatory Status | RUO |
Target | Progesterone |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA |
Sample Type | Fecal Extract,Supernatant,Urine |
Label or Dye | HRP |
Novus Biologicals™ Bovine Progesterone ELISA Kit (Colorimetric)
High sensitivity and specificity for detection of bovine progesterone.