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PCR Cloning Kits

PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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1 – 15 of 64 results
1

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Format Kit
Sample Type PCR Products
Cloning Method Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application) PCR Cloning
Vector pJET1.2/blunt
Product Line CloneJET
2

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Promotions are available
Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
3

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Promotions are available
EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Product Line TOPO™
4

Invitrogen™ Zero Blunt™ PCR Cloning Kit

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Promotions are available
Promoter T7
Content And Storage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt PCR
Bacterial or Yeast Strain TOP10
Vector Blunt DNA Cloning Vectors
Product Line Zero Blunt™
5

Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Promoter T7
Content And Storage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt PCR
Bacterial or Yeast Strain Not Included
Vector Blunt DNA Cloning Vectors
Product Line TOPO™, Zero Blunt™
6

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells

Promotions are available
Promoter T7, SP6
Content And Storage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Cloning Method TOPO™-TA
Vector TOPO-TA Vectors
Product Line TOPO™
7

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, without competent cells

Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products

Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Promoter T3, T7
Content And Storage Box 1: Store at -5 to -30°C.
• pCR XL-2-TOPO vector
• Salt Solution
• T3 Primer
• T7 Primer
• XL Control PCR Template
• XL Control PCR Primers
• Platinum SuperFi Green PCR Master Mix
• SuperFi GC Enhancer
• Nuclease-free water

Box 2: Store at room temperature.
• Gel Solubilization Buffer (L3)
• Binding Buffer (B2)
• Wash Buffer (W1)
• Elution Buffer (E1)
• PureLink Clean-up Spin Columns (in Wash Tubes)
• PureLink Recovery/Elution Tubes
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method Zero Blunt TOPO
Bacterial or Yeast Strain Not Included
For Use With (Application) PCR Cloning
Vector pCR-XL-2-TOPO
Product Line TOPO™
8

Invitrogen™ pLenti6/V5 Directional TOPO™ Cloning Kit

Directional TOPO Cloning site for rapid and efficient directional cloning of blunt-end PCR products

9

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

10

Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Promoter T7
Content And Storage TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type Cloning Kit
Cloning Method TA Cloning
Bacterial or Yeast Strain Not Included
For Use With (Application) PCR Cloning
Vector pCR2.1
Product Line TA Cloning™
11

Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

12

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Shipping Condition Dry Ice
Promoter T7, T3
Content And Storage Box 1:
• Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Vector TOPO Cloning Vectors
Product Line TOPO™
13

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10F' E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Content And Storage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10F´
Vector TOPO-TA Cloning Vectors
Product Line One Shot™
15

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Content And Storage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10
Cell Type Chemically Competent