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3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97%, Thermo Scientific Chemicals
3,3'-Diaminobenzidine tetrahydrochloride hydrate, CAS # 868272-85-9, is a peroxidase substrate that is commonly used for immunoblotting and immunohistochemical staining techniques.
95.07 € - 224.12 €
Chemical Identifiers
| CAS | 868272-85-9 |
|---|---|
| Molecular Formula | C12H18Cl4N4 |
| Molecular Weight (g/mol) | 360.10 |
| MDL Number | MFCD08273058 |
| InChI Key | KJDSORYAHBAGPP-UHFFFAOYSA-N |
| Synonym | 3,3'-diaminobenzidine tetrahydrochloride hydrate, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3'-diaminobenzidine hydrate tetrahydrochloride, biphenyl-3,3',4,4'-tetramine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride xhydrate, 3,3 inverted exclamation marka-diaminobenzidine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se |
| PubChem CID | 23191111 |
| IUPAC Name | 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate |
| SMILES | Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1 |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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15444629
|
Thermo Scientific Alfa Aesar
J62216.06 |
5 g |
95.07 €
5g |
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|
15454629
|
Thermo Scientific Alfa Aesar
|
10 g |
224.12 €
10g |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is an organic compound used as a peroxidase substrate
Application
• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is commonly used for immunoblotting and immunohistochemical staining techniques
• It has also been used for the histological stain to measure the peroxidase activity. The interaction between 3,3'-Diaminobenzidine tetrahydrochloride hydrate and peroxidase leads to the formation of a visible insoluble product. Therefore, it has been applied to measure glutamate decarboxylase activity in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces
• It is used as a chromogen for detecting the antigen-antibody complex
• This compound is also used in several laboratory applications, including electron and light microscope, and FISH (fluorescence in situ hybridization)
Chemical Identifiers
| 868272-85-9 | |
| 360.10 | |
| KJDSORYAHBAGPP-UHFFFAOYSA-N | |
| 23191111 | |
| Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1 |
| C12H18Cl4N4 | |
| MFCD08273058 | |
| 3,3'-diaminobenzidine tetrahydrochloride hydrate, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3'-diaminobenzidine hydrate tetrahydrochloride, biphenyl-3,3',4,4'-tetramine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride xhydrate, 3,3 inverted exclamation marka-diaminobenzidine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se | |
| 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate |
Specifications
| 868272-85-9 | |
| C12H18Cl4N4 | |
| 3,3'-diaminobenzidine tetrahydrochloride hydrate, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3'-diaminobenzidine hydrate tetrahydrochloride, biphenyl-3,3',4,4'-tetramine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1'-biphenyl-3,3',4,4'-tetraamine tetrahydrochloride xhydrate, 3,3 inverted exclamation marka-diaminobenzidine tetrahydrochloride hydrate, 3,3'-diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se | |
| KJDSORYAHBAGPP-UHFFFAOYSA-N | |
| 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate | |
| 23191111 | |
| Powder |
| Gray | |
| MFCD08273058 | |
| Soluble at 5%(w/v) in water | |
| Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1 | |
| 360.10 | |
| 360.11 (Anhydrous) | |
| 3,3'-Diaminobenzidine tetrahydrochloride hydrate |
Safety and Handling
GHS H Statement
H351-H315-H319-H335
Suspected of causing cancer.
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.
P201-P202-P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P308+P313-P332+P313-P362-P501c
H315-H320-H335-H341-H350
missing translation for 'tsca' : Yes
Recommended Storage : Store at -20°C
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 3,3'-Diaminobenzidine tetrahydrochloride hydrate
Signal Word
- Danger
Hazard Category
- LONG-TERM AQUATIC HAZARD Chronic 3
- Carcinogenicity Category 1B
- Germ cell mutagenicity Category 2
Hazard Statement
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P201-Obtain special instructions before use.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : 3,3-Diaminobenzidinetetrahydrochloridehydrate
RUO – Research Use Only