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Corning™ cellgro™ DMEM/Ham's F-12 Mix
Products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis. Corning™ cellgro™ DMEM/Ham's F-12 Mix support cell culture growth and substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over original formulation.
Brand: Corning™ 10-092-CM
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Description
- Modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME
- Original formulation was used to culture embryonic mouse cells
- Has been modified to support variety of normal and transformed cells, such as primary cultures of mouse and chicken cells
- Formulations can differ in amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine
- Tested to determine pH and osmolality
- Low levels of endotoxin, and absence of contamination
- Tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Chromogenic Assay
- Ham's F-12 media is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids
- Mycoplasma: Tested Negative
- Sterility: Pass
Specifications
| 2 to 8°C | |
| Liquid | |
| Glutamine, HEPES | |
| <0.25EU/mL | |
| To assess growth promotion and cytotoxicity characteristics |
| 1 X | |
| DMEM and Ham's F-12, 50/50 Mix | |
| None | |
| Inorganic Salts: 116.65mg/L CaCl2 (anhydrous), 0.417mg/L FeSO4.7H2O, 0.05mg/L Fe(NO3)3.9H2O, 311.8mg/L KCl, 0.0008mg/L CuSO4 (anhydrous), 84.95mg/L MgSO4 (anhydrous), 7000mg/L NaCl, 62.5mg/L Na2HPO4.H2O, 71mg/L Na2HPO4 (anhydrous), 2438mg/L NaHCO3, 0.4315mg/L ZnSO4.7H2O; Amino Acids: 4.45mg/L L-Alanine, 147.5mg/L L-Arginine. HCl, 7.5mg/L L-Asparagine.H2O, 6.65mg/L L-Aspartic acid, 17.56mg/L L-Cysteine.HCl.H2O, 31.285mg/L L-Cysteine.2HCl, 7.35mg/L L-Glutamic acid, 365.1mg/L L-glutamine, 18.75mg/L Glycine, 31.48mg/L L-Histidine.HCl.H2O, 54.37mg/L L-Isoleucine, 58.95mg/L L-Leucine, 91.35mg/L L-Lysine. HCl, 17.24mg/L L-Methionine, 35.48mg/L L-Phenylalanine, 17.25mg/L L-Proline, 26.25mg/L L-Serine, 53.55mg/L L-Threonine, 9.02mg/L L-Tryptophan, 55.815mg/L L-Tyrosine.2Na.2H2O, 52.85mg/L L-Valine; Vitamins: 0.00365mg/L Biotin, 2.24mg/L D-Ca-Pantothenate, 8.98mg/L Choline chloride, 2.65mg/L Folic acid, 12.61mg/L i-Inositol, 2.0185mg/L Nicotinamide, 2.031mg/L Pyridoxine. HCl, 0.219mg/L Riboflavin, 2.17mg/L Thiamine. HCl; Other: 3151mg/L D-Glucose, 2.385mg/L Hypoxanthine, Sodium , 0.105mg/L DL-Thioctic (lipoic) acid, 0.044mg/L Methyl lineoleate, 8.1mg/L Phenol red, Sodium, 0.08mg/L Putrescine.2HCl, 110mg/L Sodium pyruvate, 0.365mg/L Thymidine, 3574.8mg/L HEPES. | |
| 6 x 1 L |
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