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Molecular Probes™ CellROX™ Deep Red Flow Cytometry Assay Kit
CellROX™ Deep Red Flow Cytometry Assay Kit
Brand: Molecular Probes™ C10491
412.30 EUR valid until 2024-11-29
Use promo code "21683" to get your promotional price.
This item is not returnable.
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Description
The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).View a selection guide for all CellROX™ Reagents and Kits.
The CellROX™ Deep Red Flow Cytometry Assay Kit features:
The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.
Specifications
Mammalian Cells, Eukaryotic Cells | |
Fluorescence | |
Flow Cytometer | |
CellROX™ Deep Red Reagent | |
Tube(s) | |
100 Assays | |
DMSO (Dimethylsulfoxide) |
Contains 1 vial CellROX™ Deep Red Reagent (25 μL), 1 vial SYTOX™ Blue Dead Cell Stain (100 μL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 μL, 70% solution in water), and 1 vial DMSO (200 μL). Store kit at -5 to -30°C, protected from light. CellROX™ reagent is air sensitive. |
|
Flow Cytometry | |
Reagent | |
CellROX™ Deep Red: 644⁄665, SYTOX™ blue: 444⁄480 | |
CellROX™ | |
Wet Ice |
For Research Use Only. Not for use in diagnostic procedures.