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Gibco™ CO2 Independent Medium
A non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells.
Brand: Gibco™ 18045054
This item is not returnable.
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Description
- CO2 Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO2 incubator
- CO2 Independent Medium is ideal for transporting cells or tissue, and for the handling of mouse embryos under atmospheric conditions
- Gibco™ CO2 Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate
- A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions
- No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems
- Additionally, Gibco™ CO2 Independent Medium has been formulated with components that enhance cellular production and utilization of CO2
- Store at 2°C to 8°C, protected from light
Modified as follows
- With: phenol red, sodium pyruvate
- Without: L-glutamine, HEPES
Using CO2 Independent Medium
- CO2 Independent Medium contains no lipids or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- The CO2 Independent Medium does not require a CO2 environment to maintain physiological pH
- For maximum growth performance some cell lines may require either direct or sequential adaptation to CO2 Independent Medium
Specifications
| ISO 13485 standards, FDA | |
| 1 X | |
| CO2 Independent Medium | |
| No Glutamine, No HEPES | |
| 500 mL |
| 2°C to 8°C | |
| Liquid | |
| Phenol Red, Sodium Pyruvate | |
| Mammalian cell culture | |
| For research use only |
For Research Use Only. Not for use in diagnostic procedures.