Thermo Scientific™ Columbia Agar with Sheep Blood PLUS

Description

Isolate and cultivate fastidious microorganisms with clearly visible haemolytic reactions (Staphylococci and Streptococci) with a media formulation that contains sheep blood. Thermo Scientific™ Columbia Agar with Sheep Blood PLUS promotes haemolysis typical of Staphylococcus aureus, and it gives typical growth for Streptococcus pneumonia (dent morphology).

Grow fastidious organisms with rapid production of large colonies, clearly defined zones of haemolysis and good colonial differentiation for all-around performance. Traditionally, blood agar has been either casein hydrolysate or meat infusion media. The advantage of the first lies in the rapid production of large colonies, and the second in clearly defined zones of haemolysis and good colonial differentiation. This formulation combines the virtues of both for optimal performance.

Staphylococcus aureus is a coagulase-positive, catalase positive, gram-positive cocci that occurs singly or in pairs, tetrads, short chains, and grape-like clusters. It is commonly carried on the skin and in the nose of healthy individuals but is also a major cause of infection in both healthcare and community settings worldwide. S. aureus can produce a variety of syndromes with clinical manifestations including skin and soft tissue infections, empyema, bloodstream infections, pneumonia, osteomyelitis, septic arthritis, endocarditis, sepsis, and meningitis.

Streptococci are facultatively anaerobic, catalase-negative, gram-positive cocci that occur as pairs or chains. They cause a wide variety of clinical diseases including pharyngitis, scarlet fever, otitis media, impetigo, meningitis, neonatal sepis, pneumonia, endocarditis, osteomyelitis, and necrotizing fascitis. One of the most useful phenotypic characteristics of streptococci is the hemolytic reaction, generally classified as alpha, beta or gamma according to the appearance of zones around colonies growing on blood agar. β-hemolytic streptococci are further characterized by Lancefield groups based on the antigenic differences in group-specific polysaccharides located in the bacterial cell wall.

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