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Thermo Scientific™ GeneJET Plasmid Midiprep Kit

Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.

190.00 € - 724.00 €

Specifications

Concentration 400 ng/μL
Column Type Spin Column
Format Spin Column
Isolation Technology Silica Column
Sample Type Bacteria
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
11531645
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Thermo Scientific™
K0481
25 Preps
190.00 €
Each
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11541645
View Documents Promotion Details
Thermo Scientific™
K0482
100 Preps
724.00 €
Each
Please to purchase this item. Need a web account? Register with us today!
Description

Description

GeneJET Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights
Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture
High concentration of DNA purified— >400 ng/μL
Convenient—spin column format, centrifuge or vacuum protocols
Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
In vitro transcription
• Transfection of robust cell lines

Specifications

Specifications

400 ng/μL
Spin Column
Bacteria
Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
100-200 μg (Medium-Scale) Plasmid DNA
Plasmid MidiPrep Kit
60 min.
Spin Column
Silica Column
Midi
Not High-throughput Compatible (Manual)
GeneJET
Plasmid DNA
Videos
Safety and Handling

Safety and Handling

Product Identifier
  • GeneJET Plasmid Midiprep Kit

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor / soap
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P330-Rinse mouth.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : guanidine hydrochloride, sodium lauryl sulphate, sodium hydroxide, acetic acid,triton X-100

SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.