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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Brand: Invitrogen™ A21174

Product Code. 15270036

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Alert:

For research use only. Not for use in diagnostic procedures.

Includes:

CRISPR OFP Nuclease Vector, linearized
10X Oligonucleotide Annealing Buffer
DNase/RNase-free Water
5X Ligation Buffer
T4 DNA Ligase
U6 Forward Sequencing Primer
Control Double-stranded Oligonucleotide

This item is not returnable. View return policy

Description

Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells. The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21178).

The GeneArt™ CRISPR Nuclease Vector system offers:

• Easy-to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard to transfect or difficult to modify cell lines

For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Compliance

CoA

Recommended Storage

Store in freezer (-5 to -30°C)

Specifications

Cloning Method	Restriction Enzyme/MCS
Detection Method	Primer-probe
Format	Kit
Technique	CRISPR-Cas9
Product Type	Crispr Nuclease Vector With ofP Reporter Kit
Protein Tag	OFP fusion
Reporter Gene	OFP
For Use With (Application)	Gene Expression, Cloning
Overhang	3' overhangs

Content And Storage	Contains: • CRISPR OFP Nuclease Vector, linearized • 10X Oligonucleotide Annealing Buffer • DNase/RNase-free Water • 5X Ligation Buffer • T4 DNA Ligase • U6 Forward Sequencing Primer • Control Double-stranded Oligonucleotide Store in freezer (-5 to -30°C).
Form	Liquid
Reaction Speed	Fast
Product Line	GeneArt™
Promoter	U6, CMV
Quantity	10 reactions
Sufficient For	10 Reactions
No. of Reactions	10 Reactions
Starting Material	Oligos (DNA)

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For Research Use Only. Not for use in diagnostic procedures.

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