Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.

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Invitrogen™ PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format	Kit
Sample Type	PCR Products
Isolation Technology	Silica Spin Column
Run Time	10 min.

Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

Pricing and Availability

Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Target	DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage	The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) • ChargeSwitch™ Purification Buffer (N5) • ChargeSwitch™ Wash Buffer (W12) • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format	Kit
Product Type	PCR Clean-Up Kit
Sample Type	PCR Products
Isolation Technology	ChargeSwitch™ Chemistry
Run Time	5 min
For Use With (Application)	Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility	High-throughput Compatible
Product Line	ChargeSwitch™

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Pricing and Availability
Specifications

Content And Storage	For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format	Spin Column
Run Time	5 min.
High-throughput Compatibility	Not High-throughput Compatible (Manual)

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

Pricing and Availability

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Green Features	Fewer resources used and less waste, Less hazardous
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, TaqMan™
Detection Method	TaqMan

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2X
Green Features	Fewer resources used, less waste, less hazardous
Concentration	2X
For Use With (Application)	Gene Expression
High-throughput Compatibility	High-throughput Compatible
PCR Method	2-step RT-qPCR
Detection Method	TaqMan
Format	Tube
Product Type	Fast Advanced Cells-to-Ct Kit
Sample Type	Mammalian cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Purification Target	Lysate
Starting Material	10-100,000 Cells
Product Line	Cells-to-CT™

Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.

Pricing and Availability

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing and Availability
Specifications

Shipping Condition	Frozen, Dry Ice
GC-Rich PCR Performance	High
Content And Storage	Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Form	Liquid
Isolation Technology	Direct cell lysis
Kit Type	Direct Cell Lysis RT-qPCR
Green Features	Less hazardous, less waste
For Use With (Application)	Gene Expression
High-throughput Compatibility	Yes
PCR Method	2-step RT-qPCR
Detection Method	TaqMan
Target	cDNA
Format	Kit
Sample Type	Mammalian Cells
Reaction Speed	5 min. (Fast)
Label or Dye	ROX
Purification Target	RNA
Shelf Life	1 Year
Starting Material	10-100,000 Cells

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	Medium
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR
Polymerase	Phire Hot Start II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Tissue
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

First Strand cDNA Synthesis Kit

Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.

Pricing and Availability

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

The Ambion Single Cell-to-C_T™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Purification Time	7 min.
Green Features	Fewer resources used and less waste, less hazardous
Starting Material	1-10 Cells
For Use With (Application)	RT-PCR
PCR Method	2-step RT-qPCR
Product Line	Ambion™, Cells-to-CT™
Detection Method	TaqMan

Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit Reaction Mix (5X)

Thermo Scientific™ Maxima™ 5X Reaction Mix is a component of the Maxima First Strand cDNA Synthesis Kits for RT-qPCR (K1671/K1672/K1641/K1642) and may be purchased separately. The 5X Reaction Mix is comprised of reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers.

Pricing and Availability

Invitrogen™ TaqMan™ Gene Expression Cells-to-C_T™ Kit

The TaqMan™ Gene Expression Cells-to-C_T™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	M-MLV
Green Features	Fewer resources used and less waste, Less hazardous
PCR Method	2-step RT-qPCR
Detection Method	Primer-probe
Polymerase	DNA Polymerase
Format	Tube
Product Type	Gene Expression Cells-to-C Kit
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cell
Reaction Speed	Standard
Product Line	Ambion™, Cells-to-CT™, TaqMan™

Direct PCR Reagents and Kits

Direct PCR Reagents and Kits

Invitrogen™ PureLink™ PCR Purification Kit

Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit

Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

First Strand cDNA Synthesis Kit

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit Reaction Mix (5X)

Invitrogen™ TaqMan™ Gene Expression Cells-to-C_T™ Kit

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Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

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Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit

Invitrogen™ TaqMan™ Gene Expression Cells-to-C_T™ Kit