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Gibco™ DMEM, high glucose
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

25.45 € - 429.00 €

Specifications

Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
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Requires: Supplementation


Products 4
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10741574
GREENER_CHOICE
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Gibco™
11965092
500 mL
25.45 €
Each
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15480564
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Gibco™
11965084
1000 mL
49.17 €
1000mL
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11330882
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Gibco™
11965167
5 L
240.00 €
Each
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11340882
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Gibco™
11965175
10 L
429.00 €
Each
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Description

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With: High Glucose, L-glutamine, Phenol Red
Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Informations sur la commande

Shipping Condition: Room Temperature

Spécification

Spécification

HeLa, 293, Cos-7, and PC-12
Animal Origin-free
Liquid
Standard Serum Supplementation
High Glucose, Glutamine, Phenol Red
4500 mg/L
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)
Sterile-filtered
No HEPES, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Vidéos
Sécurité et traitement

Sécurité et traitement

missing translation for 'shelfLife' : 12 Months From Date of Manufacture

FDS
Documents

Documents

Product Certifications

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.