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Cytiva Glutathione Sepharose™ 4 Fast Flow Media
Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.
Brand: Cytiva 17-5132-01
This item is not returnable.
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Description
- Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
- Protein binding capacity: ≈10mg recombinant GST/mL media (flowrate dependent)
- Binding capacity and high flow properties allow scale-up
- Mild elution conditions preserve protein antigenicity and function
- Stable in all commonly used aqueous buffers
- pH stability: 3 to 12
Specifications
| >10 mg/mL | |
| <450 cm/hr. | |
| Highly cross-linked 4% agarose | |
| 4% Highly Cross-linked Agarose | |
| base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm | |
| 25 mL | |
| Glutathione and 10-carbon linker arm |
| Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed. | |
| 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size | |
| 3 to 12 | |
| 4°C to 30°C, 20% Ethanol | |
| 90 μm | |
| Chromatography Media |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Glutathione Sepharose 4 Fast Flow
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol