Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli

Description

One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 10¹⁰ cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids. TOP10 Electrocomp E. coli cells are transformed via electroporation and achieve higher transformation efficiencies than chemically competent cells. They can therefore be used in more challenging applications, including cloning low amounts of DNA or cDNA and gDNA library construction.

TOP10 E. coli cells are genetically similar to the DH10B strain but have been reported to be more resilient to stress conditions like osmotic shock and acidic pH stress. These cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. Similar to other DH strains, TOP10 has the lacZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal.

One Shot TOP10 Electrocomp E. coli cells offer:
• Transformation efficiencies of >1 x 10¹⁰ cfu/μg
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Easy-to-use One Shot format
TOP10 Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination.

Genotype
F^–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(Str^R) endA1 nupG

Find the strain and format that fit your needs
• We offer other DH strains in chemically competent and electrocompetent cell formats.
• Electroporation cuvettes with 0.1 cm (Cat. No. P41050), 0.2 cm (Cat. No. P45050), and 0.4 cm (Cat. No. P46050) gaps are available.
• The TOP10 strain is available in several MultiShot formats for high throughput applications.

Note: TOP10 cells do not require IPTG to induce expression from the lac promoter. A high-voltage electroporation apparatus is required.

Order Info

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