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Macherey-Nagel™ NucleoBond™ RNA Soil Mini
Easy handling and superior speed in RNA purification from soil samples for qRT-PCR analysis, as well as parallel preparation of DNA. Complete kit uses anion exchange chromatography and gravity flow columns combined with NucleoSpin Bead Tubes Type A (included).
Brand: Macherey-Nagel™ 740142.2
78.57 EUR valid until 2025-01-31
Use promo code "23894" to get your promotional price.
This item is not returnable.
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Description
NucleoBond® RNA Soil Mini kit enables efficient isolation of high quality RNA and DNA from any kind of soil or sediment sample. Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, the kit is designed with proven NucleoBond™ technology to enable high sample input and and pure nucleic acids.
- Anion exchange technology to optimize RNA yield and purity—suitable for qRT-PCR analysis
- Combination of mechanical homogenization and chemical lysis allows processing of large samples
- Parallel preparation of RNA and DNA in one hour*
- Sample disruption is achieved with NucleoSpin™ Bead Tubes Type A, optimized Lysis Buffer, and Phenol:Chloroform:Isoamylalcohol
- Optional Buffer OPT enables RNA and DNA purification from mineralic and organic soil samples
* DNA isolation requires the DNA Set for NucleoBond™ RNA Soil Mini, sold separately (man. part no. 740143.10 or 740143.50) or in a combo kit
Specifications
| Gravity Flow Column | |
| Elution volume: 50-100 μL | |
| Binding capacity: 30 μg | |
| RNA/DNA isolation from soil for qRT-PCR | |
| 2 | |
| A260/A280: 1.5 - 2.0 | |
| RNA integrity number (RIN): > 7.0 | |
| 0.25 - 2.5 μg RNA; 1.25 - 12.5 μg DNA |
| Anion Exchange Chromatography, Gravity Flow Columns, NucleoSpin Bead Tubes | |
| Soil | |
| Centrifuge for microcentrifuge tubes | |
| NucleoBond Soil Mini Columns, NucleoSpin Bead Tubes Type A, NucleoSpin RNA columns, Buffers | |
| RNA Kit + DNA Set | |
| < 0.25 - 0.5 g | |
| 60 min/12 preps (RNA); 15 min/12 preps (DNA) |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- NucleoBond RNA Soil Mini + DNA Set (2)
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Supplemental information
- MIXTURE LIST-Contains : guanidium thiocyanide, ethanol