missing translation for 'onlineSavingsMsg'
Learn More

Macherey-Nagel™ Nucleospin™ 96 Plasmid Transfection-grade

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.

1135.00 € - 5825.00 €

Specifications

Bacterial Endotoxins <50 EU/ng DNA
Content And Storage Room temperature (18 - 25 °C)
Format 96-well Plate
Isolation Technology Silica Membrane and Endotoxin-reduction Technology
Sample Type Bacterial Culture (E. coli, High-copy Plasmids)
View More Specs

Products 3
Product Code Brand No. of Samples Product Type Includes Price Quantity & Availability  
Product Code Brand No. of Samples Product Type Includes Price Quantity & Availability  
15553636
View Documents Promotion Details
Macherey-Nagel™
740492.4
4 x 96 Core Kit Filter Plates, Binding Plates, Buffers, RNase A
1135.00 €
384 reactions
Please to purchase this item. Need a web account? Register with us today!
15543636
View Documents Promotion Details
Macherey-Nagel™
740492.24
24 x 96 Core Kit Filter Plates, Binding Plates, Buffers, RNase A
5825.00 €
2304 reactions
Please to purchase this item. Need a web account? Register with us today!
15533636
View Documents Promotion Details
Macherey-Nagel™
740491.4
4 x 96 Full Kit Filter Plates, Binding Plates, MN Wash Plates, Culture Plates, Elution Plates, Buffers, RNase A
1210.00 €
384 reactions
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
  • Ultra-fast procedure simplifies the work-flow
  • High-throughput — 96-well plates allow for easy automation and supports plasmid screening
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing
Specifications

Specifications

<50 EU/ng DNA
96-well Plate
Bacterial Culture (E. coli, High-copy Plasmids)
Elution Volume: 100 - 200 μL
Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
A260/A280: 1.80 - 1.85
< 15 kbp
5 - 20 μg
Room temperature (18 - 25 °C)
Silica Membrane and Endotoxin-reduction Technology
Binding capacity: 20 μg
Transfection-grade plasmid DNA
Rapid purification of plasmids for transfection
Up to 5 mL
45 min/plate
Videos
Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : At least 1 year

Product Identifier
  • NucleoSpin 96 Plasmid T.-gr. Core (4x96)

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information
  • MIXTURE LIST-Contains : sodium hydroxide, dodecyl sulphate, guanidine hydrochloride,triethanolamine, 2-propanol, RNAse

SDS
Documents

Documents

Product Certifications