Content And Storage |
Room temperature (18 - 25 °C) |
Final Product Type |
Transfection-grade plasmid DNA |
Size |
< 15 kbp |
Isolation Technology |
Silica Membrane and Endotoxin-reduction Technology |
Purity |
A260/A280: 1.80 - 1.85 |
Sample Volume |
Up to 5 mL |
For Use With (Application) |
Rapid purification of plasmids for transfection |
Test Time |
14 min/6 preps |
Includes |
NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A |
Format |
Mini Spin Column |
For Use With (Equipment) |
Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
Efficiency |
Binding capacity: 35 μg |
Sample Type |
Bacterial Culture (E. coli, High-copy Plasmids) |
Yield |
15 - 30 μg |
Shelf Life |
At least 1 year |
Elution Volume |
Elution Volume: 30 - 50 μL |
Bacterial Endotoxins |
<50 EU/ng DNA |