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Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular, Thermo Scientific Chemicals
Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.
Brand: Thermo Scientific Acros 327111000
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Description
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0
Applications
- Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA, and RNA
- When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
- It is also used to generate DNA minisatellite fingerprints
Specifications
| Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0 | |
| DNase, RNase and Protease Free | |
| C6H6O | |
| 2927 | |
| 14, 7241 | |
| Molecular Biology | |
| 1.28 | |
| 7.8 to 8.2 (25°C) | |
| Solution |
| Yellow to Orange | |
| 136112-00-0, 67-66-3, 123-51-3 | |
| MFCD00133763 | |
| 1.2800g/mL | |
| 94.11 | |
| Glass bottle | |
| Stabilized | |
| 100 mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Skin corrosion/irritation Category 1 B
- Serious eye damage/eye irritation Category 1
- Germ cell mutagenicity Category 2
- Carcinogenicity Category 2
- Reproductive toxicity Category 2
- Specific target organ toxicity Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H331-Toxic if inhaled.
- H314-Causes severe skin burns and eye damage.
- H341-Suspected of causing genetic defects.
- H351-Suspected of causing cancer.
- H361d-Suspected of damaging the unborn child.
- H372-Causes damage to organs.
- H336-May cause drowsiness or dizziness.
- EUH066-Repeated exposure may cause skin dryness or cracking.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
Supplemental information
- MIXTURE LIST-Contains : Chloroform, Phenol,Isoamyl alcohol
RUO – Research Use Only