Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Description

Quantitatively measure lactose dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay with Thermo Scientific™ Pierce LDH Cytotoxicity Assay Kit.

Additional Information and Online Ordering

The Pierce LDH Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays.

Highlights:

• Convenient – add–mix–read assay format for adherent and suspension cells

• Colorimetric – quantitatively measures LDH release by formation of colored product

• Robust – uses stable LDH enzyme activity as a cytotoxic marker

• Flexible – ideal for high–throughput screening

• Non–radioactive – safe alternative to 51Cr–release cytotoxicity assays

Includes:

Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control

Requires:

Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96–well plate compatible with spectrophotometer, multichannel pipette

Recommended for:

• Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
• Detect cytolysis in bioreactor or 3D tissue engineering applications