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Thermo Scientific™ PolarScreen™ ER Alpha Competitor Assay, Green
Brand: Thermo Scientific™ A15882
This item is not returnable.
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Description
The PolarScreen™ ER (Estrogen Receptor) Alpha Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands. The kit uses insect cell-expressed, full-length, native (untagged) human estrogen receptor alpha (available separately, Cat. No. A15674) and a novel, high-affinity, fluorescent estrogen ligand (Fluormone ES2 Green) in a homogenous, mix-and-read assay format.This kit produces a green color read-out; a 'red' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact drugdiscoverytech@lifetech.com.
PolarScreen™ ER Alpha Competitor Assay, Green, is:
How the Assay Works
Full length ER alpha is added to a fluorescent estrogen ligand to form an ER-Fluormone ES2 complex. This complex is added to individual test compounds in multiwell plates. To determine relative affinity, the assay measures fluorescence polarization. Competing test compounds will displace the Fluormone ES2 ligand from ER alpha, permitting it to tumble rapidly and resulting in low polarization values. A shift in the fluorescence polarization value in the presence of test compound is used to determine the relative affinity of test compounds for ER alpha.
All components are manufactured under the strictest quality parameters. This product's performance, per the Certificate of Analysis, is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
Specifications
| Contains: • 4 tubes full-length ER Alpha Recombinant Protein (store at -68 to -85°C) • 1 tube of Fluormone ES2 Green Tracer (store at -5 to -30°C) • 2 bottles of ES2 Screening Buffer (store at -5 to -30°C) |
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| Fluorescent Imager | |
| 2500 Assays |
| Fluorescence | |
| 384-well Plate | |
| Competitor Assay Kit |
For Research Use Only. Not for use in diagnostic procedures.