Protein Analysis Reagents
Protein Analysis Reagents
Filtered Search Results
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
MWCO | 7.0 kDa |
---|---|
Column Type | Size-exclusion, Proprietary Resin |
Purification Target | Buffer Exchange, Protein |
Product Line | Zeba™ |
Invitrogen™ DynaMag™-2 Magnet
The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.
Cytiva Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation
Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.
Shipping Condition | Ambient temperature |
---|---|
Content And Storage | Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
Purity or Quality Grade | For Research Use Only |
Description | Recombinant Protein A covalently bound to Dynabeads |
Surface Functionality | Protein A |
Material | Polystyrene |
Concentration | 30 mg/mL |
For Use With (Application) | Immunoprecipitation |
High-throughput Compatibility | High-throughput Compatible |
Ligand Type | Protein A |
Type | Superparamagnetic Beads |
Target | Antibodies |
Format | Beads in suspension |
For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
Diameter (Metric) | 2.8 μm |
Certifications/Compliance | ISO9001 and ISO13485 |
Uniformity | Monosized 2.8 mm (CV <5%) |
Product Line | Dynabeads™ |
Thermo Scientific™ Pierce™ 2-Mercaptoethanol
Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).
Gibco™ PNGase F Glycan Cleavage Kit
PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins.
Thermo Scientific™ Pierce™ BCA Protein Assay Kits
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
---|---|
For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Invitrogen™ DynaMag™-15 Magnet
Optimized for efficient cell isolation using all types of Dynabeads™ (range 1–4.5 μm in diameter) in standard 15 mL lab tubes.
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
---|---|
Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ Pierce™ Horseradish Peroxidase
Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C. |
---|---|
Form | Powder |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Western Blotting, ELISA, Immunohistochemistry |
Product Line | Pierce™ |
Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Content And Storage | Store desiccated at -20°C, protect from moisture |
---|---|
Product Type | NHS-Fluorescein |
Label or Dye | FITC (Fluorescein) |
Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
Solubility | DMF (Dimethylformamide) |
Emission | 518 |
Excitation | 494 |
Label Type | Classic Dyes |
Chemical Reactivity | Amine |
Product Line | Pierce™ |
Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.
Shipping Condition | Ambient temperature |
---|---|
Content And Storage | Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
Purity or Quality Grade | For Research Use Only |
Description | Recombinant Protein G covalently bound to Dynabeads |
Surface Functionality | Protein G |
Material | Polystyrene |
Concentration | 30 mg/mL |
For Use With (Application) | Immunoprecipitation |
High-throughput Compatibility | High-throughput Compatible |
Ligand Type | Protein G |
Type | Superparamagnetic Beads |
Target | Antibodies |
Format | Beads in suspension |
For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
Diameter (Metric) | 2.8 μm |
Certifications/Compliance | ISO9001 and ISO13485 |
Uniformity | Monosized 2.8 mm (CV <5%) |
Product Line | Dynabeads™ |
Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.
Form | Liquid |
---|---|
Reagent Type | Detergent Solution |
Product Line | Surfact-Amps™ |
Invitrogen™ Dynabeads™ M-270 Epoxy
Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.
Shipping Condition | Room Temperature |
---|---|
Type | Epoxy Bead |
Purity or Quality Grade | Research Grade |
Format | Freeze Dried |
Surface Functionality | Epoxy |
Sample Type | Any Sample Type |
Diameter (Metric) | 2.8 μm |
Shelf Life | 24 months from date of manufacture |
Elution Volume Min | 5 μL |
Product Line | DYNAL™, Dynabeads™ |
Thermo Scientific™ Pierce™ Streptavidin
Purified recombinant streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods including western blotting, ELISA, immunohistochemistry, and fluorescence imaging.
Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C. |
---|---|
Conjugate | Unconjugated |
Target Molecule | Biotin |
Product Line | Pierce™ |