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Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

Brand: Thermo Scientific™ FD0596

280.08 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

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Product Code. 10232590

389.00 € / 250 reactions

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Description

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Thermo Scientific FastDigest NotI restriction enzyme recognizes GC^GGCCGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: CciNI.

Thermo Scientific FastDigest NotI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Spécification

Content And Storage	• FastDigest NotI (250 μL) • 10X FastDigest Buffer (1.0 mL) • 10X FastDigest Green Buffer (1.0 mL) Store at –20°C.
Enzyme	Not I
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Product Type	Restriction Enzyme
Product Line	FastDigest™
Inactivation Temperature	80°C

Methylation Sensitivity	CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature	37°C
Quantity	250 μL (250 Reactions)
Research Category	Traditional Cloning
Overhang	5' protruding ends

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Documents

Product Information

Manuals

User Guide: Reaction Set-up for Digestion of Multiple DNA Samples Version: Jan. 2015 File size: 71.4 kB

User Guide: FastDigest NotI, 250uL Version: Jan. 2015 File size: 51.8 kB

User Guide: FastDigest NotI, 50uL Version: Jan. 2015 File size: 51.7 kB

User Guide: Scaling up DNA Digestion Reaction Version: Jan. 2015 File size: 60.9 kB

User Guide: FastDigest NotI, 20uL Version: Jan. 2015 File size: 51.7 kB

User Guide: Double and Multiple Digestion of DNA Version: Jan. 2015 File size: 71.3 kB

User Guide: Fast Digestion of DNA Version: Jan. 2015 File size: 67.9 kB

Scientific Resources

Reference Materials

LabAid: FastDigest restriction enzymes File size: 62.2 kB

Product Certifications

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2863487	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberFD0596
Lot Number2925709	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberFD0596
Lot Number2967427	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberFD0596
Lot Number3018682	Certificate TypeCertificate of Analysis	Date26/01/2025	Catalog NumberFD0596
Lot Number3089135	Certificate TypeCertificate of Analysis	Date25/01/2025	Catalog NumberFD0596

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