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Thermo Scientific™ Pierce™ 660nm Protein Assay Kit
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Brand: Thermo Scientific™ 22662
174.20 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
Product Code. 10404195
This item is not returnable.
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Description
Thermo Scientific™ Pierce 660nm Protein Assay is a quick, ready- to-use colorimetric method for total protein quantitation.
Additional Information and Online Ordering
The Pierce 660nm Protein Assay is a quick, ready-to-use colorimetric method for measuring protein concentration. The assay is more linear than coomassie-based Bradford assays and compatible with higher concentrations of most detergents, reducing agents and other commonly used reagents. The accessory Ionic Detergent Compatibility Reagent (IDCR) provides for even broader detergent compatibility, making the Pierce 660nm Protein Assay the only protein assay that is suitable for samples containing Laemmli SDS sample buffer with bromophenol blue.Although the Pierce 660nm Protein Assay produces a higher level of protein-to-protein variation (37%) than other assays, such as the BCA Protein Assay, the simpler single-reagent format and broader substance compatibility make the 660nm Assay more convenient for many routine applications. The Pierce 660nm Protein Assay can be performed in either a test tube or microplate format.
The assay is based on a proprietary dye-metal complex that binds to proteins in an acidic medium. Upon binding, the reddish dye-metal complex turns to green, resulting in an absorbance shift that is measurable at wavelengths near 660nm (645 to 670). The dye interacts mainly with basic residues in proteins, such as histidine, arginine and lysine and to a lesser extent tyrosine, tryptophan and phenylalanine.
Highlights:
Versatile – works with a greater range of detergents and reducing agents than other dye-based assays
Fast – single reagent with a simple mix-and-read protocol; no working reagent to prepare
Accurate – produces standard curves that are more linear than with the Bradford assay method
Flexible – assay may be performed in test tubes or microplates
Conserve samples – requires only 10μL for microplate or 100μL for the test tube procedures
Convenient – room temperature storage means no waiting for reagent equilibration before use
Includes:
Kit contains Pierce 660nm Assay Reagent and a set of seven pre-diluted albumin protein assay standards
Specifications
| Sufficient For: 300 tube assays or 3000 microplate assays • Pierce 660 nm Protein Assay Reagent, 450 mL • Albumin Standard Pre-Diluted Set, 7 x 3.5 mL Store the assay reagent at room temperature and the pre-diluted BSA standards at 4°C. |
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| Protein Assay Kit | |
| Pierce™ | |
| Not Target-Specific | |
| Solution-based Detection, Absorbance | |
| 450 mL |
| Pierce 660nm Protein Assay Kit | |
| Colorimetric | |
| Protein Quantitation Assay | |
| 300 Tube Assays or 3000 Microplate Assays | |
| Spectrophotometer, Microplate Reader |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce 660nm Protein Assay Reagent, 450 mL.
Signal Word
- Warning
Hazard Category
- Specific target organ toxicity Category 2
Hazard Statement
- H371-May cause damage to organs.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Methyl alcohol
For Research Use Only. Not for use in diagnostic procedures.