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Molecular Probes™ Qdot™ 655 Streptavidin Conjugate

Qdot™ 655 Streptavidin Conjugate

Brand: Molecular Probes™ Q10121MP

679.44 EUR valid until 2025-04-18
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Product Code. 10591855

894.00 € / Each

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Description

Qdot™ 655 Streptavidin Conjugate comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot™ nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Qdot™ nanocrystal conjugates are provided as 1μM solutions.

Important Features of Qdot™ Streptavidin Conjugates:

Streptavidin Qdot™ 655 conjugate has emission maxima of ∼655 nm

Approximately 5 to 10 streptavidins per Qdot™ nanocrystal

Extremely photostable and bright fluorescence

Efficiently excited with single-line excitation sources

Narrow emission, large Stokes shift

Available in multiple colors

Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot™ Nanocrystals
The Qdot™ streptavidin conjugate is the size of a large macromolecule or protein (∼15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot™ streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot™ 705 and Qdot™ 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot™ colors or try the Qdot™ Streptavidin Sampler Kit, containing Qdot™ streptavidin conjugates in six colors. In addition to nanocrystal conjugates, we offer a wide range of streptavidins conjugated to Alexa Fluor™ dyes, Oregon Green™ dye, enzyme conjugates, and traditional fluorophores like Texas Red™ dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.

Use the primary antibody search tool to find biotinylated primary antibodies

Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Specifications

Concentration	1 μM
Quantity	200 μL
Shipping Condition	Room Temperature
Product Line	Qdot™

Conjugate	Qdot 655
Content And Storage	Store in refrigerator (2–8°C).
Form	Liquid
Product Type	Streptavidin Conjugate (fluorescent)

Product Suggestions Documents

Documents

Scientific Resources

Application Notes

Application Note: Optimization of Qdot® nanocrystals for flow cytometry File size: 2.6 MB

Product Information

Manuals

Qdot Streptavidin Conjugates Version: 09-13-2007 File size: 431.5 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2831248	Certificate TypeCertificate of Analysis	Date14/03/2024	Catalog NumberQ10121MP
Lot Number2663880	Certificate TypeCertificate of Analysis	Date18/09/2023	Catalog NumberQ10121MP
Lot Number2486542	Certificate TypeCertificate of Analysis	Date09/06/2022	Catalog NumberQ10121MP
Lot Number2354407	Certificate TypeCertificate of Analysis	Date15/06/2021	Catalog NumberQ10121MP
Lot Number2305180	Certificate TypeCertificate of Analysis	Date12/01/2021	Catalog NumberQ10121MP

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For Research Use Only. Not for use in diagnostic procedures.

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Molecular Probes™ Qdot™ 655 Streptavidin Conjugate

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