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Thermo Scientific™ Tango™ EDG4-bla U2OS Cells

Brand: Thermo Scientific™ K1442

Product Code. 10721824

13350.00 € / Each

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Estimated Shipment: 02-04-2025
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Description

The Tango™ EDG4-bla U2OS cells contain the human Endothelial Differentiation Gene 4 (EDG4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ EDG4-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of LPA (18:1). In addition, Tango™ EDG4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

Specifications

Accession Number	NM_004720
Cell State	Dividing Cells
Cytoplasmic Tail	Tail from AVPR2 GPCR
For Use With (Application)	GPCRs
Includes	EDG4-bla U2OS cells, EDG4-bla U2OS Assay Protocol, Certificate of Analysis
Product Line	Tango™
Reporter Gene	BLA (Beta-Lactamase)
Type	Dividing Cells
System Type	Tango™

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ EDG4-bla U2OS cells • Tango™ EDG4-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ EDG4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method	Fluorescence
Gene Symbol	EDG4
Population Status	Pool of Stable Cells
Quantity	2 x 10⁶ Cells
Target Entry	EDG4
Cell Line	U2OS
Druggable Target	GPCR

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Lot Number

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Lot Number	Certificate Type	Date	Catalog Number
Lot Number1458354	Certificate TypeCertificate of Analysis	Date16/04/2017	Catalog NumberK1442
Lot Number40501	Certificate TypeCertificate of Analysis	Date16/04/2017	Catalog NumberK1442

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Thermo Scientific™ Tango™ EDG4-bla U2OS Cells

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